DOI: 10.1073/pnas.150238697. Bacterial-type DNA Holliday junction resolvases in eukaryotic viruses

Bacterial-type DNA Holliday junction resolvases in eukaryotic viruses

10.1073/pnas.150238697

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Homologous DNA recombination promotes genetic diversity and the maintenance of genome integrity, yet no enzymes with specificity for the Holliday junction (HJ)—a key DNA recombination intermediate—have been purified and characterized from metazoa or their viruses. Here we identify critical structural elements of RuvC, a bacterial HJ resolvase, in uncharacterized open reading frames from poxviruses and an iridovirus. The putative vaccinia virus resolvase was expressed as a recombinant protein, affinity purified, and shown to specifically bind and cleave a synthetic HJ to yield nicked duplex molecules. Mutation of either of two conserved acidic amino acids abrogated the catalytic activity of the A22R protein without affecting HJ binding. The presence of bacterial-type enzymes in metazoan viruses raises evolutionary questions.

Bibliography

Garcia, A. D., Aravind, L., Koonin, E. V., & Moss, B. (2000). Bacterial-type DNA Holliday junction resolvases in eukaryotic viruses. Proceedings of the National Academy of Sciences, 97(16), 8926â8931.

Authors 4

Alonzo D. Garcia (first)
L. Aravind (additional)
Eugene V. Koonin (additional)
Bernard Moss (additional)

References 44 Referenced 79

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:32 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 11:37 p.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 6:49 a.m.)
Issued	25 years, 1 month ago (July 11, 2000)
Published	25 years, 1 month ago (July 11, 2000)
Published Online	25 years, 1 month ago (July 11, 2000)
Published Print	25 years, 1 month ago (Aug. 1, 2000)

Funders 0

None

BibTeX

@article{Garcia_2000, title={Bacterial-type DNA Holliday junction resolvases in eukaryotic viruses}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.150238697}, DOI={10.1073/pnas.150238697}, number={16}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Garcia, Alonzo D. and Aravind, L. and Koonin, Eugene V. and Moss, Bernard}, year={2000}, month=jul, pages={8926–8931} }

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