DOI: 10.1073/pnas.142309999. Self-assembling peptide hydrogel fosters chondrocyte extracellular matrix production and cell division: Implications for cartilage tissue repair

Self-assembling peptide hydrogel fosters chondrocyte extracellular matrix production and cell division: Implications for cartilage tissue repair

10.1073/pnas.142309999

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Emerging medical technologies for effective and lasting repair of articular cartilage include delivery of cells or cell-seeded scaffolds to a defect site to initiate de novo tissue regeneration. Biocompatible scaffolds assist in providing a template for cell distribution and extracellular matrix (ECM) accumulation in a three-dimensional geometry. A major challenge in choosing an appropriate scaffold for cartilage repair is the identification of a material that can simultaneously stimulate high rates of cell division and high rates of cell synthesis of phenotypically specific ECM macromolecules until repair evolves into steady-state tissue maintenance. We have devised a self-assembling peptide hydrogel scaffold for cartilage repair and developed a method to encapsulate chondrocytes within the peptide hydrogel. During 4 weeks of culture in vitro , chondrocytes seeded within the peptide hydrogel retained their morphology and developed a cartilage-like ECM rich in proteoglycans and type II collagen, indicative of a stable chondrocyte phenotype. Time-dependent accumulation of this ECM was paralleled by increases in material stiffness, indicative of deposition of mechanically functional neo-tissue. Taken together, these results demonstrate the potential of a self-assembling peptide hydrogel as a scaffold for the synthesis and accumulation of a true cartilage-like ECM within a three-dimensional cell culture for cartilage tissue repair.

Bibliography

Kisiday, J., Jin, M., Kurz, B., Hung, H., Semino, C., Zhang, S., & Grodzinsky, A. J. (2002). Self-assembling peptide hydrogel fosters chondrocyte extracellular matrix production and cell division: Implications for cartilage tissue repair. Proceedings of the National Academy of Sciences, 99(15), 9996â10001.

Authors 7

J. Kisiday (first)
M. Jin (additional)
B. Kurz (additional)
H. Hung (additional)
C. Semino (additional)
S. Zhang (additional)
A. J. Grodzinsky (additional)

References 42 Referenced 917

Dates

Type	When
Created	23 years, 1 month ago (July 28, 2002, 6:26 p.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:13 a.m.)
Indexed	6 days, 1 hour ago (Aug. 30, 2025, 12:18 p.m.)
Issued	23 years, 1 month ago (July 15, 2002)
Published	23 years, 1 month ago (July 15, 2002)
Published Online	23 years, 1 month ago (July 15, 2002)
Published Print	23 years, 1 month ago (July 23, 2002)

Funders 0

None

BibTeX

@article{Kisiday_2002, title={Self-assembling peptide hydrogel fosters chondrocyte extracellular matrix production and cell division: Implications for cartilage tissue repair}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.142309999}, DOI={10.1073/pnas.142309999}, number={15}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kisiday, J. and Jin, M. and Kurz, B. and Hung, H. and Semino, C. and Zhang, S. and Grodzinsky, A. J.}, year={2002}, month=jul, pages={9996–10001} }

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