DOI: 10.1073/pnas.142224299. VEGF receptor 2 and the adherens junction as a mechanical transducer in vascular endothelial cells

VEGF receptor 2 and the adherens junction as a mechanical transducer in vascular endothelial cells

10.1073/pnas.142224299

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Blood-flow interactions with the vascular endothelium represents a specialized example of mechanical regulation of cell function that has important physiological and pathophysiological cardiovascular consequences. Yet, the mechanisms of mechanostransduction are not understood fully. This study shows that shear stress induces a rapid induction as well as nuclear translocation of the vascular endothelial growth factor (VEGF) receptor 2 and promotes the binding of the VEGF receptor 2 and the adherens junction molecules, VE-cadherin and β-catenin, to the endothelial cytoskeleton. These changes are accompanied by the formation of a complex containing the VEGF receptor 2–VE-cadherin–β-catenin. In endothelial cells lacking VE-cadherin, shear stress did not augment nuclear translocation of the VEGF receptor 2 and phosphorylation of Akt1 and P38 as well as transcriptional induction of a reporter gene regulated by a shear stress-responsive promoter. These results suggest that VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells.

Bibliography

Shay-Salit, A., Shushy, M., Wolfovitz, E., Yahav, H., Breviario, F., Dejana, E., & Resnick, N. (2002). VEGF receptor 2 and the adherens junction as a mechanical transducer in vascular endothelial cells. Proceedings of the National Academy of Sciences, 99(14), 9462â9467.

Authors 7

Ayelet Shay-Salit (first)
Moran Shushy (additional)
Efrat Wolfovitz (additional)
Hava Yahav (additional)
Ferruccio Breviario (additional)
Elisabetta Dejana (additional)
Nitzan Resnick (additional)

References 40 Referenced 271

Dates

Type	When
Created	23 years, 1 month ago (July 28, 2002, 6:26 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 11:50 p.m.)
Indexed	1 month ago (July 26, 2025, 4:25 a.m.)
Issued	23 years, 2 months ago (June 21, 2002)
Published	23 years, 2 months ago (June 21, 2002)
Published Online	23 years, 2 months ago (June 21, 2002)
Published Print	23 years, 1 month ago (July 9, 2002)

Funders 0

None

BibTeX

@article{Shay_Salit_2002, title={VEGF receptor 2 and the adherens junction as a mechanical transducer in vascular endothelial cells}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.142224299}, DOI={10.1073/pnas.142224299}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Shay-Salit, Ayelet and Shushy, Moran and Wolfovitz, Efrat and Yahav, Hava and Breviario, Ferruccio and Dejana, Elisabetta and Resnick, Nitzan}, year={2002}, month=jun, pages={9462–9467} }

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