DOI: 10.1073/pnas.1418377111. Volta potential phase plate for in-focus phase contrast transmission electron microscopy

Volta potential phase plate for in-focus phase contrast transmission electron microscopy

10.1073/pnas.1418377111

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Significance Biological electron cryomicroscopy is limited by the radiation sensitivity of the samples and the consequent need to minimize exposure to the beam. This, in turn, results in low-contrast images with a poor signal-to-noise ratio. The current practice to improve phase contrast by defocusing results in contrast transfer functions necessitating image restoration to provide interpretable data. Phase plates enable in-focus phase contrast, but the existing ones, including the thin film Zernike-type phase plate, suffer from severe limitations, such as a short usable life span, fringing artifacts, and problems in using them in automated data acquisition procedures. The Volta phase plate presented here solves those problems and has the potential to become a practical solution for in-focus phase contrast in transmission electron microscopy.

Bibliography

Danev, R., Buijsse, B., Khoshouei, M., Plitzko, J. M., & Baumeister, W. (2014). Volta potential phase plate for in-focus phase contrast transmission electron microscopy. Proceedings of the National Academy of Sciences, 111(44), 15635â15640.

Authors 5

Radostin Danev (first)
Bart Buijsse (additional)
Maryam Khoshouei (additional)
Jürgen M. Plitzko (additional)
Wolfgang Baumeister (additional)

References 25 Referenced 469

Dates

Type	When
Created	10 years, 10 months ago (Oct. 21, 2014, 2:45 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:08 a.m.)
Indexed	3 weeks ago (Aug. 2, 2025, 12:56 a.m.)
Issued	10 years, 10 months ago (Oct. 20, 2014)
Published	10 years, 10 months ago (Oct. 20, 2014)
Published Online	10 years, 10 months ago (Oct. 20, 2014)
Published Print	10 years, 9 months ago (Nov. 4, 2014)

Funders 0

None

BibTeX

@article{Danev_2014, title={Volta potential phase plate for in-focus phase contrast transmission electron microscopy}, volume={111}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1418377111}, DOI={10.1073/pnas.1418377111}, number={44}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Danev, Radostin and Buijsse, Bart and Khoshouei, Maryam and Plitzko, Jürgen M. and Baumeister, Wolfgang}, year={2014}, month=oct, pages={15635–15640} }

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