DOI: 10.1073/pnas.1406744111. Initial bridges between two ribosomal subunits are formed within 9.4 milliseconds, as studied by time-resolved cryo-EM

Initial bridges between two ribosomal subunits are formed within 9.4 milliseconds, as studied by time-resolved cryo-EM

10.1073/pnas.1406744111

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Significance The protein-synthesizing machinery of the cell, the ribosome, is made up of two subunits, which in bacteria are held together by 12 molecular bridges. Understanding the mechanism and timeline of bridge formation is crucial to understanding the mechanism of bacterial translation initiation. To study the timeline of bridge formation, we developed microfluidic devices that mix two interacting components, spray the mixture onto an electron microscopy grid, and flash freeze the grid for a minimum reaction time of 9.4 ms. This study shows for the first time to the authors' knowledge that eight of the 12 bridges form within 9.4 ms, whereas the remaining four bridges take longer than 43 ms to form.

Bibliography

Shaikh, T. R., Yassin, A. S., Lu, Z., Barnard, D., Meng, X., Lu, T.-M., Wagenknecht, T., & Agrawal, R. K. (2014). Initial bridges between two ribosomal subunits are formed within 9.4 milliseconds, as studied by time-resolved cryo-EM. Proceedings of the National Academy of Sciences, 111(27), 9822â9827.

Authors 8

Tanvir R. Shaikh (first)
Aymen S. Yassin (additional)
Zonghuan Lu (additional)
David Barnard (additional)
Xing Meng (additional)
Toh-Ming Lu (additional)
Terence Wagenknecht (additional)
Rajendra K. Agrawal (additional)

References 44 Referenced 41

Dates

Type	When
Created	11 years, 2 months ago (June 24, 2014, 4:39 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 10:50 p.m.)
Indexed	1 month, 2 weeks ago (July 16, 2025, 9:41 a.m.)
Issued	11 years, 2 months ago (June 23, 2014)
Published	11 years, 2 months ago (June 23, 2014)
Published Online	11 years, 2 months ago (June 23, 2014)
Published Print	11 years, 1 month ago (July 8, 2014)

Funders 0

None

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  "abstract": "<jats:title>Significance</jats:title>\n          <jats:p>The protein-synthesizing machinery of the cell, the ribosome, is made up of two subunits, which in bacteria are held together by 12 molecular bridges. Understanding the mechanism and timeline of bridge formation is crucial to understanding the mechanism of bacterial translation initiation. To study the timeline of bridge formation, we developed microfluidic devices that mix two interacting components, spray the mixture onto an electron microscopy grid, and flash freeze the grid for a minimum reaction time of 9.4 ms. This study shows for the first time to the authors' knowledge that eight of the 12 bridges form within 9.4 ms, whereas the remaining four bridges take longer than 43 ms to form.</jats:p>",
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