Taura syndrome virus IRES initiates translation by binding its tRNA-mRNA–like structural element in the ribosomal decoding center
10.1073/pnas.1406335111
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

Significance Ribosomes decode genetic information encoded in mRNAs to synthesize cellular proteins. Initiation of translation is a key step, during which the ORF coding for a protein gets properly positioned on the ribosome with the AUG start codon and its cognate tRNA located in the ribosomal peptidyl site. Here, we report molecular structures of a eukaryotic ribosome complexed with viral mRNA, which uncover an unusual mechanism of initiation. The structures reveal that viral mRNAs carrying an intergenic RNA structure known as the internal ribosome entry site (IRES) initiate translation by binding a tRNA-mRNA–like element in the aminoacyl site of the ribosome. A structural mechanism of how viral mRNAs with intergenic IRESs hijack host ribosomes is proposed.

Bibliography

Koh, C. S., Brilot, A. F., Grigorieff, N., & Korostelev, A. A. (2014). Taura syndrome virus IRES initiates translation by binding its tRNA-mRNA–like structural element in the ribosomal decoding center. Proceedings of the National Academy of Sciences, 111(25), 9139–9144.

Authors 4
  1. Cha San Koh (first)
  2. Axel F. Brilot (additional)
  3. Nikolaus Grigorieff (additional)
  4. Andrei A. Korostelev (additional)
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Dates
Type When
Created 11 years, 2 months ago (June 10, 2014, 3:46 a.m.)
Deposited 3 years, 2 months ago (June 7, 2022, 7:29 a.m.)
Indexed 1 month, 2 weeks ago (July 2, 2025, 2:35 p.m.)
Issued 11 years, 2 months ago (June 9, 2014)
Published 11 years, 2 months ago (June 9, 2014)
Published Online 11 years, 2 months ago (June 9, 2014)
Published Print 11 years, 1 month ago (June 24, 2014)
Funders 0

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@article{Koh_2014, title={Taura syndrome virus IRES initiates translation by binding its tRNA-mRNA–like structural element in the ribosomal decoding center}, volume={111}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1406335111}, DOI={10.1073/pnas.1406335111}, number={25}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Koh, Cha San and Brilot, Axel F. and Grigorieff, Nikolaus and Korostelev, Andrei A.}, year={2014}, month=jun, pages={9139–9144} }