DOI: 10.1073/pnas.1332299100. Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation

Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation

10.1073/pnas.1332299100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Heterochromatin formation in yeast involves deacetylation of histones, but the precise relationship between acetylation and the association of proteins such as Sir3, Sir4, and the histone deacetylase Sir2 with chromatin is still unclear. Here we show that Sir3 protein spreads to subtelomeric DNA in cells lacking the transcription-related histone acetyltransferases GCN5 and ELP3 . Spreading correlates with hypoacetylation of lysines in the histone H3 tail and results in deacetylation of lysine 16 in histone H4. De-repression of genes situated very close to the ends of the chromosomes in gcn5 elp3 suggests that Sir3 spreads into subtelomeric DNA from the tip of the telomere. Interestingly, growth defects caused by gcn5 elp3 mutation can be suppressed by SIR deletion, suggesting that Sir proteins become detrimental for growth when chromatin is severely hypoacetylated.

Bibliography

Kristjuhan, A., Wittschieben, B. Ã., Walker, J., Roberts, D., Cairns, B. R., & Svejstrup, J. Q. (2003). Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation. Proceedings of the National Academy of Sciences, 100(13), 7551â7556.

Authors 6

Arnold Kristjuhan (first)
Birgitte Ø. Wittschieben (additional)
Jane Walker (additional)
Douglas Roberts (additional)
Bradley R. Cairns (additional)
Jesper Q. Svejstrup (additional)

References 34 Referenced 33

Dates

Type	When
Created	22 years, 2 months ago (June 24, 2003, 1:47 p.m.)
Deposited	3 years, 4 months ago (April 25, 2022, 9:24 p.m.)
Indexed	2 months ago (July 2, 2025, 3:08 p.m.)
Issued	22 years, 2 months ago (June 9, 2003)
Published	22 years, 2 months ago (June 9, 2003)
Published Online	22 years, 2 months ago (June 9, 2003)
Published Print	22 years, 2 months ago (June 24, 2003)

Funders 0

None

BibTeX

@article{Kristjuhan_2003, title={Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1332299100}, DOI={10.1073/pnas.1332299100}, number={13}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kristjuhan, Arnold and Wittschieben, Birgitte Ø. and Walker, Jane and Roberts, Douglas and Cairns, Bradley R. and Svejstrup, Jesper Q.}, year={2003}, month=jun, pages={7551–7556} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        2
      ]
    ],
    "date-time": "2025-07-02T19:08:00Z",
    "timestamp": 1751483280440
  },
  "reference-count": 34,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "13",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2003,
        6,
        24
      ]
    ]
  },
  "abstract": "<jats:p>\n            Heterochromatin formation in yeast involves deacetylation of histones, but  the precise relationship between acetylation and the association of proteins  such as Sir3, Sir4, and the histone deacetylase Sir2 with chromatin is still  unclear. Here we show that Sir3 protein spreads to subtelomeric DNA in cells  lacking the transcription-related histone acetyltransferases\n            <jats:italic>GCN5</jats:italic>\n            and\n            <jats:italic>ELP3</jats:italic>\n            . Spreading correlates with hypoacetylation of lysines in the  histone H3 tail and results in deacetylation of lysine 16 in histone H4.  De-repression of genes situated very close to the ends of the chromosomes in\n            <jats:italic>gcn5 elp3</jats:italic>\n            suggests that Sir3 spreads into subtelomeric DNA from the  tip of the telomere. Interestingly, growth defects caused by\n            <jats:italic>gcn5  elp3</jats:italic>\n            mutation can be suppressed by\n            <jats:italic>SIR</jats:italic>\n            deletion, suggesting that  Sir proteins become detrimental for growth when chromatin is severely  hypoacetylated.\n          </jats:p>",
  "DOI": "10.1073/pnas.1332299100",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        6,
        24
      ]
    ],
    "date-time": "2003-06-24T17:47:39Z",
    "timestamp": 1056476859000
  },
  "page": "7551-7556",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 33,
  "title": "Spreading of Sir3 protein in cells with severe histone H3  hypoacetylation",
  "prefix": "10.1073",
  "volume": "100",
  "author": [
    {
      "given": "Arnold",
      "family": "Kristjuhan",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Mechanisms of Transcription Laboratory, Cancer  Research UK London Research Institute, Clare Hall Laboratories, South Mimms,  Hertfordshire EN6 3LD, United Kingdom; and  Howard Hughes Medical Institute and Department  of Oncological Science, Huntsman Cancer Institute, University of Utah, Salt  Lake City, UT 84112"
        }
      ]
    },
    {
      "given": "Birgitte \u00d8.",
      "family": "Wittschieben",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Mechanisms of Transcription Laboratory, Cancer  Research UK London Research Institute, Clare Hall Laboratories, South Mimms,  Hertfordshire EN6 3LD, United Kingdom; and  Howard Hughes Medical Institute and Department  of Oncological Science, Huntsman Cancer Institute, University of Utah, Salt  Lake City, UT 84112"
        }
      ]
    },
    {
      "given": "Jane",
      "family": "Walker",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Mechanisms of Transcription Laboratory, Cancer  Research UK London Research Institute, Clare Hall Laboratories, South Mimms,  Hertfordshire EN6 3LD, United Kingdom; and  Howard Hughes Medical Institute and Department  of Oncological Science, Huntsman Cancer Institute, University of Utah, Salt  Lake City, UT 84112"
        }
      ]
    },
    {
      "given": "Douglas",
      "family": "Roberts",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Mechanisms of Transcription Laboratory, Cancer  Research UK London Research Institute, Clare Hall Laboratories, South Mimms,  Hertfordshire EN6 3LD, United Kingdom; and  Howard Hughes Medical Institute and Department  of Oncological Science, Huntsman Cancer Institute, University of Utah, Salt  Lake City, UT 84112"
        }
      ]
    },
    {
      "given": "Bradley R.",
      "family": "Cairns",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Mechanisms of Transcription Laboratory, Cancer  Research UK London Research Institute, Clare Hall Laboratories, South Mimms,  Hertfordshire EN6 3LD, United Kingdom; and  Howard Hughes Medical Institute and Department  of Oncological Science, Huntsman Cancer Institute, University of Utah, Salt  Lake City, UT 84112"
        }
      ]
    },
    {
      "given": "Jesper Q.",
      "family": "Svejstrup",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Mechanisms of Transcription Laboratory, Cancer  Research UK London Research Institute, Clare Hall Laboratories, South Mimms,  Hertfordshire EN6 3LD, United Kingdom; and  Howard Hughes Medical Institute and Department  of Oncological Science, Huntsman Cancer Institute, University of Utah, Salt  Lake City, UT 84112"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        2003,
        6,
        9
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_2_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(01)00259-8"
    },
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81159-9"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0959-437X(98)80146-9"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81160-5"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.16.8.4349"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.7.4.592"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(01)00301-X"
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.8.19.2257"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90512-X"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.94.6.2186"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.11.1.83"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35001622"
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.110148297"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.97.12.6658"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(02)00647-0"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90584-9"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(00)80179-3"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/19.12.3060"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.274.5290.1201"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(01)00219-2"
    },
    {
      "key": "e_1_3_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/38444"
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)54150-8"
    },
    {
      "key": "e_1_3_2_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.10.10.1247"
    },
    {
      "key": "e_1_3_2_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M008668200"
    },
    {
      "key": "e_1_3_2_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M110532200"
    },
    {
      "key": "e_1_3_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/383092a0"
    },
    {
      "key": "e_1_3_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/ng1017"
    },
    {
      "key": "e_1_3_2_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/ng993"
    },
    {
      "key": "e_1_3_2_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/17.19.5679"
    },
    {
      "key": "e_1_3_2_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0955-0674(97)80011-7"
    },
    {
      "key": "e_1_3_2_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/20.3.520"
    },
    {
      "key": "e_1_3_2_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(02)00759-6"
    },
    {
      "key": "e_1_3_2_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.1001502"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.1332299100",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        26
      ]
    ],
    "date-time": "2022-04-26T01:24:20Z",
    "timestamp": 1650936260000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.1332299100"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2003,
        6,
        9
      ]
    ]
  },
  "references-count": 34,
  "journal-issue": {
    "issue": "13",
    "published-print": {
      "date-parts": [
        [
          2003,
          6,
          24
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.1332299100"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.1332299100",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        2003,
        6,
        9
      ]
    ]
  },
  "assertion": [
    {
      "value": "2002-11-18",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2003-06-09",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}