DOI: 10.1073/pnas.132468999. An ectopic human <i>XIST</i> gene can induce chromosome inactivation in postdifferentiation human HT-1080 cells

An ectopic human XIST gene can induce chromosome inactivation in postdifferentiation human HT-1080 cells

10.1073/pnas.132468999

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

It has been believed that XIST RNA requires a discrete window in early development to initiate the series of chromatin-remodeling events that form the heterochromatic inactive X chromosome. Here we investigate four adult male HT-1080 fibrosarcoma cell lines expressing ectopic human XIST and demonstrate that these postdifferentiation cells can undergo chromosomal inactivation outside of any normal developmental context. All four clonal lines inactivated the transgene-containing autosome to varying degrees and with variable stability. One clone in particular consistently localized the ectopic XIST RNA to a discrete chromosome territory that exhibited striking hallmarks of inactivation, including long-range transcriptional inactivation. Results suggest that some postdifferentiation cell lines are capable of de novo chromosomal inactivation; however, long-term retention of autosomal inactivation was less common, which suggests that autosomal inactivation may confer a selective disadvantage. These results have fundamental significance for understanding genomic programming in early development.

Bibliography

Hall, L. L., Byron, M., Sakai, K., Carrel, L., Willard, H. F., & Lawrence, J. B. (2002). An ectopic human XIST gene can induce chromosome inactivation in postdifferentiation human HT-1080 cells. Proceedings of the National Academy of Sciences, 99(13), 8677â8682.

Authors 6

Lisa L. Hall (first)
Meg Byron (additional)
Kosuke Sakai (additional)
Laura Carrel (additional)
Huntington F. Willard (additional)
Jeanne B. Lawrence (additional)

References 38 Referenced 110

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R Tam, C Johnson, L Shopland, J McNeil, J B Lawrence Applications of RNA FISH For Visualizing Gene Expression and Nuclear Architecture (Oxford Univ. Press, Oxford, 2002). / Applications of RNA FISH For Visualizing Gene Expression and Nuclear Architecture by Tam R (2002)
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Dates

Type	When
Created	23 years, 1 month ago (July 28, 2002, 6:26 p.m.)
Deposited	3 years, 4 months ago (April 25, 2022, 8:45 p.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 3:07 p.m.)
Issued	23 years, 2 months ago (June 18, 2002)
Published	23 years, 2 months ago (June 18, 2002)
Published Online	23 years, 2 months ago (June 18, 2002)
Published Print	23 years, 2 months ago (June 25, 2002)

Funders 0

None

BibTeX

@article{Hall_2002, title={An ectopic human XIST gene can induce chromosome inactivation in postdifferentiation human HT-1080 cells}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.132468999}, DOI={10.1073/pnas.132468999}, number={13}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hall, Lisa L. and Byron, Meg and Sakai, Kosuke and Carrel, Laura and Willard, Huntington F. and Lawrence, Jeanne B.}, year={2002}, month=jun, pages={8677–8682} }

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