Comprehensive structural model of the mechanochemical cycle of a mitotic motor highlights molecular adaptations in the kinesin family
10.1073/pnas.1319848111
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

Significance Kinesins are a superfamily of ATP-dependent motors that are important for a wide variety of microtubule-based functions in eukaryotic cells. Kinesins have evolved to allow variable tuning of their motor properties, but the link between molecular variation and motor function is largely unknown. To understand this link, we have studied an essential mitotic kinesin, kinesin-5, which is the target of anticancer drugs. We used cryo-electron microscopy to visualize directly sequential conformational changes of structural elements during the motor ATPase cycle. We have identified the contribution of kinesin-5–specific variations to motor function indicating that kinesins indeed are precisely tuned according to cellular function. This insight will be important in designing kinesin-specific inhibitors in different disease contexts.

Bibliography

Goulet, A., Major, J., Jun, Y., Gross, S. P., Rosenfeld, S. S., & Moores, C. A. (2014). Comprehensive structural model of the mechanochemical cycle of a mitotic motor highlights molecular adaptations in the kinesin family. Proceedings of the National Academy of Sciences, 111(5), 1837–1842.

Authors 6
  1. Adeline Goulet (first)
  2. Jennifer Major (additional)
  3. Yonggun Jun (additional)
  4. Steven P. Gross (additional)
  5. Steven S. Rosenfeld (additional)
  6. Carolyn A. Moores (additional)
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Dates
Type When
Created 11 years, 6 months ago (Jan. 21, 2014, 11:02 p.m.)
Deposited 3 years, 2 months ago (June 7, 2022, 8:06 a.m.)
Indexed 2 months, 2 weeks ago (June 2, 2025, 8:34 p.m.)
Issued 11 years, 7 months ago (Jan. 21, 2014)
Published 11 years, 7 months ago (Jan. 21, 2014)
Published Online 11 years, 7 months ago (Jan. 21, 2014)
Published Print 11 years, 6 months ago (Feb. 4, 2014)
Funders 0

None

@article{Goulet_2014, title={Comprehensive structural model of the mechanochemical cycle of a mitotic motor highlights molecular adaptations in the kinesin family}, volume={111}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1319848111}, DOI={10.1073/pnas.1319848111}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Goulet, Adeline and Major, Jennifer and Jun, Yonggun and Gross, Steven P. and Rosenfeld, Steven S. and Moores, Carolyn A.}, year={2014}, month=jan, pages={1837–1842} }