DOI: 10.1073/pnas.1313368111. High throughput 3D super-resolution microscopy reveals <i>Caulobacter crescentus</i> in vivo Z-ring organization

High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization

10.1073/pnas.1313368111

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Significance The bacterial cytoskeletal protein FtsZ, which forms a constricting “Z-ring” during cell division, is the major cytoskeletal protein involved in cell division in almost all prokaryotes, and is a key next-generation antibiotic target. However, the small size of the Z-ring, approximately 500 nm in diameter, makes it difficult to observe in vivo. We provide a quantitative nanoscale picture of Z-ring organization in live cells; these results improve our understanding of the structural and force-generating roles of FtsZ in bacterial cell division. To achieve this, we created an automated modality of superresolution fluorescence microscopy, allowing high-throughput live cell microscopy at nanoscale resolution; this technique should be broadly useful in prokaryotic and eukaryotic systems.

Bibliography

Holden, S. J., Pengo, T., Meibom, K. L., Fernandez Fernandez, C., Collier, J., & Manley, S. (2014). High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization. Proceedings of the National Academy of Sciences, 111(12), 4566â4571.

Authors 6

Seamus J. Holden (first)
Thomas Pengo (additional)
Karin L. Meibom (additional)
Carmen Fernandez Fernandez (additional)
Justine Collier (additional)
Suliana Manley (additional)

References 29 Referenced 201

Dates

Type	When
Created	11 years, 5 months ago (March 10, 2014, 9:34 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 10:55 p.m.)
Indexed	3 weeks, 4 days ago (Aug. 5, 2025, 8:56 a.m.)
Issued	11 years, 5 months ago (March 10, 2014)
Published	11 years, 5 months ago (March 10, 2014)
Published Online	11 years, 5 months ago (March 10, 2014)
Published Print	11 years, 5 months ago (March 25, 2014)

Funders 0

None

BibTeX

@article{Holden_2014, title={High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization}, volume={111}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1313368111}, DOI={10.1073/pnas.1313368111}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Holden, Seamus J. and Pengo, Thomas and Meibom, Karin L. and Fernandez Fernandez, Carmen and Collier, Justine and Manley, Suliana}, year={2014}, month=mar, pages={4566–4571} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        5
      ]
    ],
    "date-time": "2025-08-05T12:56:08Z",
    "timestamp": 1754398568452
  },
  "reference-count": 29,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "12",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2014,
        3,
        25
      ]
    ]
  },
  "abstract": "<jats:title>Significance</jats:title>\n          <jats:p>The bacterial cytoskeletal protein FtsZ, which forms a constricting \u201cZ-ring\u201d during cell division, is the major cytoskeletal protein involved in cell division in almost all prokaryotes, and is a key next-generation antibiotic target. However, the small size of the Z-ring, approximately 500 nm in diameter, makes it difficult to observe in vivo. We provide a quantitative nanoscale picture of Z-ring organization in live cells; these results improve our understanding of the structural and force-generating roles of FtsZ in bacterial cell division. To achieve this, we created an automated modality of superresolution fluorescence microscopy, allowing high-throughput live cell microscopy at nanoscale resolution; this technique should be broadly useful in prokaryotic and eukaryotic systems.</jats:p>",
  "DOI": "10.1073/pnas.1313368111",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2014,
        3,
        11
      ]
    ],
    "date-time": "2014-03-11T01:34:20Z",
    "timestamp": 1394501660000
  },
  "page": "4566-4571",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 201,
  "title": "High throughput 3D super-resolution microscopy reveals\n            <i>Caulobacter crescentus</i>\n            in vivo Z-ring organization",
  "prefix": "10.1073",
  "volume": "111",
  "author": [
    {
      "given": "Seamus J.",
      "family": "Holden",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Laboratory of Experimental Biophysics, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne, CH-1015 Lausanne, Switzerland; and"
        }
      ]
    },
    {
      "given": "Thomas",
      "family": "Pengo",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Laboratory of Experimental Biophysics, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne, CH-1015 Lausanne, Switzerland; and"
        }
      ]
    },
    {
      "given": "Karin L.",
      "family": "Meibom",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Laboratory of Experimental Biophysics, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne, CH-1015 Lausanne, Switzerland; and"
        }
      ]
    },
    {
      "given": "Carmen",
      "family": "Fernandez Fernandez",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, CH-1015 Lausanne, Switzerland"
        }
      ]
    },
    {
      "given": "Justine",
      "family": "Collier",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, CH-1015 Lausanne, Switzerland"
        }
      ]
    },
    {
      "given": "Suliana",
      "family": "Manley",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Laboratory of Experimental Biophysics, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne, CH-1015 Lausanne, Switzerland; and"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        2014,
        3,
        10
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_3_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cub.2005.06.038"
    },
    {
      "key": "e_1_3_3_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/emboj.2009.277"
    },
    {
      "key": "e_1_3_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1239248"
    },
    {
      "key": "e_1_3_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0404439101"
    },
    {
      "key": "e_1_3_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1159961"
    },
    {
      "key": "e_1_3_3_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/354161a0"
    },
    {
      "key": "e_1_3_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.micro.57.012903.074300"
    },
    {
      "key": "e_1_3_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M009033200"
    },
    {
      "key": "e_1_3_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.bpj.2012.05.035"
    },
    {
      "key": "e_1_3_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.emboj.7601895"
    },
    {
      "key": "e_1_3_3_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1153529"
    },
    {
      "key": "e_1_3_3_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1529/biophysj.107.120345"
    },
    {
      "key": "e_1_3_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1371/journal.pone.0012680"
    },
    {
      "key": "e_1_3_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/mmi.12331"
    },
    {
      "key": "e_1_3_3_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1371/journal.pbio.1001389"
    },
    {
      "key": "e_1_3_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cphc.201100686"
    },
    {
      "key": "e_1_3_3_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.copbio.2007.07.007"
    },
    {
      "key": "e_1_3_3_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1127344"
    },
    {
      "key": "e_1_3_3_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2011.07579.x"
    },
    {
      "key": "e_1_3_3_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.2038911"
    },
    {
      "key": "e_1_3_3_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.01419-07"
    },
    {
      "key": "e_1_3_3_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/nn4012847"
    },
    {
      "key": "e_1_3_3_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth.1202"
    },
    {
      "key": "e_1_3_3_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1574-6976.2007.00082.x"
    },
    {
      "key": "e_1_3_3_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi201669d"
    },
    {
      "key": "e_1_3_3_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.1222254110"
    },
    {
      "key": "e_1_3_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.052595099"
    },
    {
      "key": "e_1_3_3_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.186.17.5775-5781.2004"
    },
    {
      "key": "e_1_3_3_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2011.07677.x"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.1313368111",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T02:55:22Z",
    "timestamp": 1649818522000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.1313368111"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2014,
        3,
        10
      ]
    ]
  },
  "references-count": 29,
  "journal-issue": {
    "issue": "12",
    "published-print": {
      "date-parts": [
        [
          2014,
          3,
          25
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.1313368111"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.1313368111",
  "relation": {
    "has-review": [
      {
        "id-type": "doi",
        "id": "10.3410/f.718305870.793518148",
        "asserted-by": "object"
      }
    ]
  },
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        2014,
        3,
        10
      ]
    ]
  },
  "assertion": [
    {
      "value": "2014-03-10",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}