Structure and assembly of an inner membrane platform for initiation of type IV pilus biogenesis
10.1073/pnas.1312313110
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

Significance Type IV pili are long, thin fibers, formed mainly of polymers of a single pilin protein, which are displayed on the surfaces of many bacteria, including several human pathogens. Here, we report three-dimensional reconstructions of the PilMNO inner membrane complex, alone and in complex with pilin protein, through a combination of X-ray crystallography and electron microscopy. PilMNO forms a dimeric T-shaped structure, binding two copies of the pilin protein at its extremities. The results provide a structural model for the way in which pilin is harvested from the inner membrane and made available to other components of the type IV pilus biogenesis machinery.

Bibliography

Karuppiah, V., Collins, R. F., Thistlethwaite, A., Gao, Y., & Derrick, J. P. (2013). Structure and assembly of an inner membrane platform for initiation of type IV pilus biogenesis. Proceedings of the National Academy of Sciences, 110(48).

Authors 5
  1. Vijaykumar Karuppiah (first)
  2. Richard F. Collins (additional)
  3. Angela Thistlethwaite (additional)
  4. Ya Gao (additional)
  5. Jeremy P. Derrick (additional)
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Dates
Type When
Created 11 years, 9 months ago (Nov. 11, 2013, 11:48 p.m.)
Deposited 3 years, 2 months ago (June 7, 2022, 6:34 a.m.)
Indexed 3 weeks, 5 days ago (Aug. 3, 2025, 7:03 p.m.)
Issued 11 years, 9 months ago (Nov. 11, 2013)
Published 11 years, 9 months ago (Nov. 11, 2013)
Published Online 11 years, 9 months ago (Nov. 11, 2013)
Published Print 11 years, 9 months ago (Nov. 26, 2013)
Funders 0

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@article{Karuppiah_2013, title={Structure and assembly of an inner membrane platform for initiation of type IV pilus biogenesis}, volume={110}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1312313110}, DOI={10.1073/pnas.1312313110}, number={48}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Karuppiah, Vijaykumar and Collins, Richard F. and Thistlethwaite, Angela and Gao, Ya and Derrick, Jeremy P.}, year={2013}, month=nov }