DOI: 10.1073/pnas.130181797. True optical resolution beyond the Rayleigh limit achieved by standing wave illumination

True optical resolution beyond the Rayleigh limit achieved by standing wave illumination

10.1073/pnas.130181797

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

During the last decade, various efforts have been undertaken to enhance the resolution of optical microscopes, mostly because of their importance in biological sciences. Herein, we describe a method to increase the resolution of fluorescence microscopy by illuminating the specimen with a mesh-like interference pattern of a laser source and electronic postprocessing of the images. We achieve 100-nm optical resolution, an improvement by a factor of more than 2 compared with standard fluorescence microscopy and of 1.5 compared with confocal scanning.

Bibliography

Frohn, J. T., Knapp, H. F., & Stemmer, A. (2000). True optical resolution beyond the Rayleigh limit achieved by standing wave illumination. Proceedings of the National Academy of Sciences, 97(13), 7232â7236.

Authors 3

Jan T. Frohn (first)
Helmut F. Knapp (additional)
Andreas Stemmer (additional)

References 28 Referenced 198

M Born, E Wolf Principles of Optics (Pergamon, New York, 1980). / Principles of Optics by Born M (1980)
10.1080/713819421
10.1111/j.1365-2818.1979.tb01178.x
10.1046/j.1365-2818.1998.00290.x
10.1364/AO.34.003576
J W Goodman Introduction to Fourier Optics (McGraw–Hill, New York, 1996). / Introduction to Fourier Optics by Goodman J W (1996)
10.1364/JOSA.57.000056
10.1364/AO.24.000194
V Krishnamurthi, B Bailey, F Lanni Proc SPIE Int Soc Opt Eng 2655, 18–25 (1996). / Proc SPIE Int Soc Opt Eng by Krishnamurthi V (1996)
10.1364/JOSA.56.001463
10.1364/AO.38.007245
10.1364/OL.25.000046
10.1007/978-1-4757-5348-6_11
10.1080/09500349314552011
10.1007/978-1-4757-5348-6_3
D A Agard, Y Hiraoka, P Shaw, J W Sedat Methods in Cell Biology, eds D L Taylor, Y-L Wang (Academic, San Diego) 30, 353–377 (1989). / Methods in Cell Biology by Agard D A (1989)
S Inoué Methods in Cell Biology, eds D L Taylor, Y-L Wang (Academic, San Diego) 30, 85–112 (1989). / Methods in Cell Biology by Inoué S (1989)
10.1086/BBLv181n2p336
10.1002/1361-6374(199312)1:4<187::AID-BIO1>3.3.CO;2-G
G J Brakenhoff, E A van Spronsen, H T M van der Voort, N Nanninga Methods in Cell Biology, eds D L Taylor, Y-L Wang (Academic, San Diego) 30, 379–398 (1989). / Methods in Cell Biology by Brakenhoff G J (1989)
10.1364/JOSAA.9.002159
10.1038/366044a0
10.1046/j.1365-2818.1997.2290789.x
10.1046/j.1365-2818.1999.00576.x
10.1364/OL.22.001905
10.1016/S0030-4018(98)00210-7
T Wilson, M A A Neil, R Jus̆kaitis Proc SPIE Int Soc Opt Eng 3261, 4–6 (1998). / Proc SPIE Int Soc Opt Eng by Wilson T (1998)
10.1007/978-1-4615-5859-0

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 10:03 p.m.)
Indexed	2 months ago (June 26, 2025, 6:45 a.m.)
Issued	25 years, 2 months ago (June 6, 2000)
Published	25 years, 2 months ago (June 6, 2000)
Published Online	25 years, 2 months ago (June 6, 2000)
Published Print	25 years, 2 months ago (June 20, 2000)

Funders 0

None

BibTeX

@article{Frohn_2000, title={True optical resolution beyond the Rayleigh limit achieved by standing wave illumination}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.130181797}, DOI={10.1073/pnas.130181797}, number={13}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Frohn, Jan T. and Knapp, Helmut F. and Stemmer, Andreas}, year={2000}, month=jun, pages={7232–7236} }

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