DOI: 10.1073/pnas.1233634100. Conjugation to gold nanoparticles enhances polyethylenimine's transfer of plasmid DNA into mammalian cells

Conjugation to gold nanoparticles enhances polyethylenimine's transfer of plasmid DNA into mammalian cells

10.1073/pnas.1233634100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Branched polyethylenimine (PEI) chains with an average molecular mass of 2 kDa (PEI2) have been covalently attached to gold nanoparticles (GNPs), and the potency of the resulting PEI2–GNPs conjugates as vectors for the delivery of plasmid DNA into monkey kidney (COS-7) cells in the presence of serum in vitro has been systematically investigated. The transfection efficiencies vary as a function of the PEI/gold molar ratio in the conjugates, with the best one (PEI2–GNPII) being 12 times more potent than the unmodified polycation. This potency can be further doubled by adding amphiphilic N -dodecyl–PEI2 during complex formation with DNA. The resulting ternary complexes are at least 1 order of magnitude more efficient than the 25-kDa PEI, one of the premier polycationic gene-delivery vectors. Importantly, although unmodified PEI2 transfects just 4% of the cells, PEI2–GNPII transfects 25%, and the PEI2–GNPII/dodecyl–PEI2 ternary complex transfects 50% of the cells. The intracellular trafficking of the DNA complexes of these vectors, monitored by transmission electron microscopy, has detected the complexes in the nucleus <1 h after transfection.

Bibliography

Thomas, M., & Klibanov, A. M. (2003). Conjugation to gold nanoparticles enhances polyethylenimineâs transfer of plasmid DNA into mammalian cells. Proceedings of the National Academy of Sciences, 100(16), 9138â9143.

Authors 2

Mini Thomas (first)
Alexander M. Klibanov (additional)

References 44 Referenced 487

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Dates

Type	When
Created	22 years ago (Aug. 5, 2003, 1:14 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 10:03 p.m.)
Indexed	3 days, 12 hours ago (Aug. 23, 2025, 9:31 p.m.)
Issued	22 years ago (July 28, 2003)
Published	22 years ago (July 28, 2003)
Published Online	22 years ago (July 28, 2003)
Published Print	22 years ago (Aug. 5, 2003)

Funders 0

None

BibTeX

@article{Thomas_2003, title={Conjugation to gold nanoparticles enhances polyethylenimine’s transfer of plasmid DNA into mammalian cells}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1233634100}, DOI={10.1073/pnas.1233634100}, number={16}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Thomas, Mini and Klibanov, Alexander M.}, year={2003}, month=jul, pages={9138–9143} }

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