DOI: 10.1073/pnas.1233536100. A four-way junction accelerates hairpin ribozyme folding via a discrete intermediate

A four-way junction accelerates hairpin ribozyme folding via a discrete intermediate

10.1073/pnas.1233536100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The natural form of the hairpin ribozyme comprises two major structural elements: a four-way RNA junction and two internal loops carried by adjacent arms of the junction. The ribozyme folds into its active conformation by an intimate association between the loops, and the efficiency of this process is greatly enhanced by the presence of the junction. We have used single-molecule spectroscopy to show that the natural form fluctuates among three distinct states: the folded state and two additional, rapidly interconverting states (proximal and distal) that are inherited from the junction. The proximal state juxtaposes the two loop elements, thereby increasing the probability of their interaction and thus accelerating folding by nearly three orders of magnitude and allowing the ribozyme to fold rapidly in physiological conditions. Therefore, the hairpin ribozyme exploits the dynamics of the junction to facilitate the formation of the active site from its other elements. Dynamic interplay between structural elements, as we demonstrate for the hairpin ribozyme, may be a general theme for other functional RNA molecules.

Bibliography

Tan, E., Wilson, T. J., Nahas, M. K., Clegg, R. M., Lilley, D. M. J., & Ha, T. (2003). A four-way junction accelerates hairpin ribozyme folding via a discrete intermediate. Proceedings of the National Academy of Sciences, 100(16), 9308â9313.

Authors 6

Elliot Tan (first)
Timothy J. Wilson (additional)
Michelle K. Nahas (additional)
Robert M. Clegg (additional)
David M. J. Lilley (additional)
Taekjip Ha (additional)

References 28 Referenced 176

Dates

Type	When
Created	22 years ago (Aug. 5, 2003, 1:14 p.m.)
Deposited	3 years, 4 months ago (April 25, 2022, 9:29 p.m.)
Indexed	1 year ago (Aug. 12, 2024, 7:15 a.m.)
Issued	22 years, 1 month ago (July 25, 2003)
Published	22 years, 1 month ago (July 25, 2003)
Published Online	22 years, 1 month ago (July 25, 2003)
Published Print	22 years ago (Aug. 5, 2003)

Funders 0

None

BibTeX

@article{Tan_2003, title={A four-way junction accelerates hairpin ribozyme folding via a discrete intermediate}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1233536100}, DOI={10.1073/pnas.1233536100}, number={16}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Tan, Elliot and Wilson, Timothy J. and Nahas, Michelle K. and Clegg, Robert M. and Lilley, David M. J. and Ha, Taekjip}, year={2003}, month=jul, pages={9308–9313} }

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