DOI: 10.1073/pnas.1230660100. Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus

Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus

10.1073/pnas.1230660100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

RRS1-R confers broad-spectrum resistance to several strains of the causal agent of bacterial wilt, Ralstonia solanacearum . Although genetically defined as recessive, this R gene encodes a protein whose structure combines the TIR-NBS-LRR domains found in several R proteins and a WRKY motif characteristic of some plant transcriptional factors and behaves as a dominant gene in transgenic susceptible plants. Here we show that PopP2, a R. solanacearum type III effector, which belongs to the YopJ/AvrRxv protein family, is the avirulence protein recognized by RRS1-R. Furthermore, an interaction between PopP2 and both RRS1-R and RRS1-S, present in the resistant Nd-1 and susceptible Col-5 Arabidopsis thaliana ecotypes, respectively, was detected by using the yeast split-ubiquitin two-hybrid system. This interaction, which required the full-length R protein, was not observed between the RRS1 proteins and PopP1, another member of the YopJ/AvrRxv family present in strain GMI1000 and that confers avirulence in Petunia . We further demonstrate that both the Avr protein and the RRS1 proteins colocalize in the nucleus and that the nuclear localization of the RRS1 proteins are dependent on the presence of PopP2.

Bibliography

Deslandes, L., Olivier, J., Peeters, N., Feng, D. X., Khounlotham, M., Boucher, C., Somssich, I., Genin, S., & Marco, Y. (2003). Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus. Proceedings of the National Academy of Sciences, 100(13), 8024â8029.

Authors 9

Laurent Deslandes (first)
Jocelyne Olivier (additional)
Nemo Peeters (additional)
Dong Xin Feng (additional)
Manirath Khounlotham (additional)
Christian Boucher (additional)
Imre Somssich (additional)
Stéphane Genin (additional)
Yves Marco (additional)

References 41 Referenced 569

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Dates

Type	When
Created	22 years, 2 months ago (June 24, 2003, 1:47 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 10:10 p.m.)
Indexed	3 weeks, 2 days ago (Aug. 7, 2025, 4:52 a.m.)
Issued	22 years, 2 months ago (June 3, 2003)
Published	22 years, 2 months ago (June 3, 2003)
Published Online	22 years, 2 months ago (June 3, 2003)
Published Print	22 years, 2 months ago (June 24, 2003)

Funders 0

None

BibTeX

@article{Deslandes_2003, title={Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1230660100}, DOI={10.1073/pnas.1230660100}, number={13}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Deslandes, Laurent and Olivier, Jocelyne and Peeters, Nemo and Feng, Dong Xin and Khounlotham, Manirath and Boucher, Christian and Somssich, Imre and Genin, Stéphane and Marco, Yves}, year={2003}, month=jun, pages={8024–8029} }

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  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
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}