DOI: 10.1073/pnas.1120027109. Engineering rotor ring stoichiometries in the ATP synthase

Engineering rotor ring stoichiometries in the ATP synthase

10.1073/pnas.1120027109

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

ATP synthase membrane rotors consist of a ring of c-subunits whose stoichiometry is constant for a given species but variable across different ones. We investigated the importance of c/c-subunit contacts by site-directed mutagenesis of a conserved stretch of glycines (GxGxGxGxG) in a bacterial c 11 ring. Structural and biochemical studies show a direct, specific influence on the c-subunit stoichiometry, revealing c <11 , c 12 , c 13 , c 14 , and c >14 rings. Molecular dynamics simulations rationalize this effect in terms of the energetics and geometry of the c-subunit interfaces. Quantitative data from a spectroscopic interaction study demonstrate that the complex assembly is independent of the c-ring size. Real-time ATP synthesis experiments in proteoliposomes show the mutant enzyme, harboring the larger c 12 instead of c 11 , is functional at lower ion motive force. The high degree of compliance in the architecture of the ATP synthase rotor offers a rationale for the natural diversity of c-ring stoichiometries, which likely reflect adaptations to specific bioenergetic demands. These results provide the basis for bioengineering ATP synthases with customized ion-to-ATP ratios, by sequence modifications.

Bibliography

Pogoryelov, D., Klyszejko, A. L., Krasnoselska, G. O., Heller, E.-M., Leone, V., Langer, J. D., Vonck, J., MÃ¼ller, D. J., Faraldo-GÃ³mez, J. D., & Meier, T. (2012). Engineering rotor ring stoichiometries in the ATP synthase. Proceedings of the National Academy of Sciences, 109(25).

Authors 10

Denys Pogoryelov (first)
Adriana L. Klyszejko (additional)
Ganna O. Krasnoselska (additional)
Eva-Maria Heller (additional)
Vanessa Leone (additional)
Julian D. Langer (additional)
Janet Vonck (additional)
Daniel J. Müller (additional)
José D. Faraldo-Gómez (additional)
Thomas Meier (additional)

References 65 Referenced 80

Dates

Type	When
Created	13 years, 3 months ago (May 25, 2012, 6:47 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 9:28 p.m.)
Indexed	1 month ago (July 25, 2025, 6:02 a.m.)
Issued	13 years, 3 months ago (May 24, 2012)
Published	13 years, 3 months ago (May 24, 2012)
Published Online	13 years, 3 months ago (May 24, 2012)
Published Print	13 years, 2 months ago (June 19, 2012)

Funders 0

None

BibTeX

@article{Pogoryelov_2012, title={Engineering rotor ring stoichiometries in the ATP synthase}, volume={109}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1120027109}, DOI={10.1073/pnas.1120027109}, number={25}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Pogoryelov, Denys and Klyszejko, Adriana L. and Krasnoselska, Ganna O. and Heller, Eva-Maria and Leone, Vanessa and Langer, Julian D. and Vonck, Janet and Müller, Daniel J. and Faraldo-Gómez, José D. and Meier, Thomas}, year={2012}, month=may }

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