DOI: 10.1073/pnas.111581598. Selection for <i>in vivo</i> regulators of bacterial virulence

Selection for in vivo regulators of bacterial virulence

10.1073/pnas.111581598

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We devised a noninvasive genetic selection strategy to identify positive regulators of bacterial virulence genes during actual infection of an intact animal host. This strategy combines random mutagenesis with a switch-like reporter of transcription that confers antibiotic resistance in the off state and sensitivity in the on state. Application of this technology to the human intestinal pathogen Vibrio cholerae identified several regulators of cholera toxin and a central virulence gene regulator that are operative during infection. These regulators function in chemotaxis, signaling pathways, transport across the cell envelope, biosynthesis, and adherence. We show that phenotypes that appear genetically independent in cell culture become interrelated in the host milieu.

Bibliography

Lee, S. H., Butler, S. M., & Camilli, A. (2001). Selection for in vivo regulators of bacterial virulence. Proceedings of the National Academy of Sciences, 98(12), 6889â6894.

Authors 3

Sang Ho Lee (first)
Susan M. Butler (additional)
Andrew Camilli (additional)

References 25 Referenced 177

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:44 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 8:39 p.m.)
Indexed	3 hours, 56 minutes ago (Aug. 28, 2025, 8:01 a.m.)
Issued	24 years, 2 months ago (June 5, 2001)
Published	24 years, 2 months ago (June 5, 2001)
Published Online	24 years, 2 months ago (June 5, 2001)
Published Print	24 years, 2 months ago (June 5, 2001)

Funders 0

None

BibTeX

@article{Lee_2001, title={Selection for in vivo regulators of bacterial virulence}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.111581598}, DOI={10.1073/pnas.111581598}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Lee, Sang Ho and Butler, Susan M. and Camilli, Andrew}, year={2001}, month=jun, pages={6889–6894} }

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