DOI: 10.1073/pnas.111153798. RNA editing activity is associated with splicing factors in lnRNP particles: The nuclear pre-mRNA processing machinery

RNA editing activity is associated with splicing factors in lnRNP particles: The nuclear pre-mRNA processing machinery

10.1073/pnas.111153798

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Multiple members of the ADAR (adenosine deaminases acting on RNA) gene family are involved in A-to-I RNA editing. It has been speculated that they may form a large multicomponent protein complex. Possible candidates for such complexes are large nuclear ribonucleoprotein (lnRNP) particles. The lnRNP particles consist mainly of four spliceosomal subunits that assemble together with the pre-mRNA to form a large particle and thus are viewed as the naturally assembled pre-mRNA processing machinery. Here we investigated the presence of ADARs in lnRNP particles by Western blot analysis using anti-ADAR antibodies and by indirect immunoprecipitation. Both ADAR1 and ADAR2 were found associated with the spliceosomal components Sm and SR proteins within the lnRNP particles. The two ADARs, associated with lnRNP particles, were enzymatically active in site-selective A-to-I RNA editing. We demonstrate the association of ADAR RNA editing enzymes with physiological supramolecular complexes, the lnRNP particles.

Bibliography

Raitskin, O., Cho, D.-S. C., Sperling, J., Nishikura, K., & Sperling, R. (2001). RNA editing activity is associated with splicing factors in lnRNP particles: The nuclear pre-mRNA processing machinery. Proceedings of the National Academy of Sciences, 98(12), 6571â6576.

Authors 5

Oleg Raitskin (first)
Dan-Sung C. Cho (additional)
Joseph Sperling (additional)
Kazuko Nishikura (additional)
Ruth Sperling (additional)

References 48 Referenced 80

B L Bass, K Nishikura, W Keller, P H Seeburg, R B Emeson, M A O'Connell, C E Sameul, A Herbert RNA 3, 947–949 (1997). / RNA by Bass B L (1997)
H C Smith, J M Gott, M R Hanson RNA 3, 1105–1123 (1997). / RNA by Smith H C (1997)
A Gerber, M A O'Connell, W Keller RNA 3, 453–463 (1997). / RNA by Gerber A (1997)
10.1073/pnas.91.24.11457
10.1128/MCB.17.5.2413
10.1038/379460a0
10.1074/jbc.271.50.31795
10.1128/MCB.15.3.1389
10.1128/MCB.15.10.5376
10.1017/S1355838200000170
10.1016/0092-8674(91)90568-J
10.1126/science.7992055
10.1038/387303a0
10.1074/jbc.274.14.9472
10.1046/j.1471-4159.2000.741290.x
10.1093/emboj/17.4.1120
10.1126/science.290.5497.1765
10.1002/j.1460-2075.1996.tb00331.x
10.1006/jmbi.1994.0081
R Sperling, J Sperling The Eukaryotic Nucleus, Molecular Biochemistry and Macromolecular Assemblies, eds P R Strauss, S H Wilson (Telford, Caldwell, NJ) 2, 453–476 (1990). / The Eukaryotic Nucleus, Molecular Biochemistry and Macromolecular Assemblies by Sperling R (1990)
R Sperling, J Sperling RNP Particles, Splicing, and Autoimmune Diseases, ed J Schenkel (Springer, Berlin), pp. 29–47 (1998). (10.1007/978-3-642-80356-7_2) / RNP Particles, Splicing, and Autoimmune Diseases by Sperling R (1998)
10.1073/pnas.93.17.8830
10.1146/annurev.bi.65.070196.002055
J M Moore, C C Query, P A Sharp The RNA World, eds R F Gesteland, J F Atkins (Cold Spring Harbor Lab. Press, Plainview, NY), pp. 303–358 (1993). / The RNA World by Moore J M (1993)
10.1016/S0955-0674(97)80003-8
10.1006/jmbi.1997.0898
10.1006/jsbi.1997.3926
10.1006/jmbi.1998.2078
10.1073/pnas.93.5.1875
10.1016/0092-8674(93)90622-W
10.1016/0003-2697(91)90534-Z
10.1016/S0021-9258(17)36857-6
10.2144/98245bm15
10.1073/pnas.83.18.6721
10.1073/pnas.78.5.2737
10.1083/jcb.111.6.2217
10.1101/gad.6.5.837
10.1073/pnas.86.2.466
10.1128/mcb.5.3.569-575.1985
10.1093/nar/22.15.3084
10.1083/jcb.144.4.603
10.1046/j.1471-4159.1997.69010043.x
10.1074/jbc.270.29.17098
T W Nilsen RNA Structure and Function, eds R W Simons, M Grunberg-Manago (Cold Spring Harbor Lab. Press, Plainview, NY), pp. 279–307 (1998). / RNA Structure and Function by Nilsen T W (1998)
C B Burge, T H Tuschl, P A Sharp The RNA World, eds R F Gesteland, T R Cech, J F Atkins (Cold Spring Harbor Lab. Press, 2nd Ed., Plainview, NY), pp. 525–560 (1999). / The RNA World by Burge C B (1999)
10.1146/annurev.ge.28.120194.000245
10.1016/S0092-8674(00)80568-1
10.1016/S0896-6273(00)80878-8

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:44 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 8:38 p.m.)
Indexed	3 months, 1 week ago (May 26, 2025, 5:06 a.m.)
Issued	24 years, 3 months ago (May 29, 2001)
Published	24 years, 3 months ago (May 29, 2001)
Published Online	24 years, 3 months ago (May 29, 2001)
Published Print	24 years, 3 months ago (June 5, 2001)

Funders 0

None

BibTeX

@article{Raitskin_2001, title={RNA editing activity is associated with splicing factors in lnRNP particles: The nuclear pre-mRNA processing machinery}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.111153798}, DOI={10.1073/pnas.111153798}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Raitskin, Oleg and Cho, Dan-Sung C. and Sperling, Joseph and Nishikura, Kazuko and Sperling, Ruth}, year={2001}, month=may, pages={6571–6576} }

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