Remodeling of actin filaments by ADF/cofilin proteins
10.1073/pnas.1110109108
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

Cofilin/ADF proteins play key roles in the dynamics of actin, one of the most abundant and highly conserved eukaryotic proteins. We used cryoelectron microscopy to generate a 9-Å resolution three-dimensional reconstruction of cofilin-decorated actin filaments, the highest resolution achieved for a complex of F-actin with an actin-binding protein. We show that the cofilin-induced change in the filament twist is due to a unique conformation of the actin molecule unrelated to any previously observed state. The changes between the actin protomer in naked F-actin and in the actin-cofilin filament are greater than the conformational changes between G- and F-actin. Our results show the structural plasticity of actin, suggest that other actin-binding proteins may also induce large but different conformational changes, and show that F-actin cannot be described by a single molecular model.

Bibliography

Galkin, V. E., Orlova, A., Kudryashov, D. S., Solodukhin, A., Reisler, E., Schröder, G. F., & Egelman, E. H. (2011). Remodeling of actin filaments by ADF/cofilin proteins. Proceedings of the National Academy of Sciences, 108(51), 20568–20572.

Authors 7
  1. Vitold E. Galkin (first)
  2. Albina Orlova (additional)
  3. Dmitri S. Kudryashov (additional)
  4. Alexander Solodukhin (additional)
  5. Emil Reisler (additional)
  6. Gunnar F. Schröder (additional)
  7. Edward H. Egelman (additional)
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Dates
Type When
Created 13 years, 8 months ago (Dec. 8, 2011, 8:38 p.m.)
Deposited 3 years, 2 months ago (June 7, 2022, 4:17 a.m.)
Indexed 1 day, 16 hours ago (Aug. 30, 2025, 12:54 p.m.)
Issued 13 years, 8 months ago (Dec. 7, 2011)
Published 13 years, 8 months ago (Dec. 7, 2011)
Published Online 13 years, 8 months ago (Dec. 7, 2011)
Published Print 13 years, 8 months ago (Dec. 20, 2011)
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@article{Galkin_2011, title={Remodeling of actin filaments by ADF/cofilin proteins}, volume={108}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1110109108}, DOI={10.1073/pnas.1110109108}, number={51}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Galkin, Vitold E. and Orlova, Albina and Kudryashov, Dmitri S. and Solodukhin, Alexander and Reisler, Emil and Schröder, Gunnar F. and Egelman, Edward H.}, year={2011}, month=dec, pages={20568–20572} }