DOI: 10.1073/pnas.1030497100. TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution

TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution

10.1073/pnas.1030497100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In Saccharomyces cerevisiae Mediator, a subgroup of proteins (Srb8, Srb9, Srb10, and Srb11) form a module, which is involved in negative regulation of transcription. Homologues of Srb10 and Srb11 are found in some mammalian Mediator preparations, whereas no clear homologues have been reported for Srb8 and Srb9. Here, we identify a TRAP240/ARC250 homologue in Schizosaccharomyces pombe and demonstrate that this protein, spTrap240, is stably associated with a larger form of Mediator, which also contains conserved homologues of Srb8, Srb10, and Srb11. We find that spTrap240 and Sch. pombe Srb8 (spSrb8) regulate the same distinct subset of genes and have indistinguishable phenotypic characteristics. Importantly, Mediator containing the spSrb8/spTrap240/spSrb10/spSrb11 subunits is isolated only in free form, devoid of RNA polymerase II. In contrast, Mediator lacking this module associates with the polymerase. Our findings provide experimental evidence for recent suggestions that TRAP230/ARC240 and TRAP240/ARC250 may indeed be the Srb8 and Srb9 homologues of mammalian Mediator. Apparently Srb8/TRAP230/ARC240, Srb9/TRAP240/ARC250, Srb10, and Srb11 constitute a conserved Mediator submodule, which is involved in negative regulation of transcription in all eukaryotes.

Bibliography

Samuelsen, C. O., Baraznenok, V., Khorosjutina, O., SpÃ¥hr, H., Kieselbach, T., Holmberg, S., & Gustafsson, C. M. (2003). TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution. Proceedings of the National Academy of Sciences, 100(11), 6422â6427.

Authors 7

Camilla O. Samuelsen (first)
Vera Baraznenok (additional)
Olga Khorosjutina (additional)
Henrik Spåhr (additional)
Thomas Kieselbach (additional)
Steen Holmberg (additional)
Claes M. Gustafsson (additional)

References 36 Referenced 103

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Dates

Type	When
Created	22 years, 2 months ago (May 27, 2003, 1:10 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 8 p.m.)
Indexed	4 months, 2 weeks ago (April 8, 2025, 5:39 p.m.)
Issued	22 years, 3 months ago (May 8, 2003)
Published	22 years, 3 months ago (May 8, 2003)
Published Online	22 years, 3 months ago (May 8, 2003)
Published Print	22 years, 3 months ago (May 27, 2003)

Funders 0

None

BibTeX

@article{Samuelsen_2003, title={TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1030497100}, DOI={10.1073/pnas.1030497100}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Samuelsen, Camilla O. and Baraznenok, Vera and Khorosjutina, Olga and Spåhr, Henrik and Kieselbach, Thomas and Holmberg, Steen and Gustafsson, Claes M.}, year={2003}, month=may, pages={6422–6427} }

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