DOI: 10.1073/pnas.102168299. Structure and function correlation in histone H2A peptide-mediated gene transfer

Structure and function correlation in histone H2A peptide-mediated gene transfer

10.1073/pnas.102168299

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Histone H2A has been found to be efficient in DNA delivery into a number of cell lines. We have reasoned that this DNA-delivery activity is mediated by two mechanisms: ( i ) electrostatically driven DNA binding and condensation by histone and ( ii ) nuclear import of these histone H2A⋅DNA polyplexes via nuclear localization signals in the protein. We have identified a 37-aa N-terminal peptide of histone H2A that is active in in vitro gene transfer. This peptide can function as a nuclear localization signal and can bind DNA. Amino acid substitutions that replace positively charged residues and/or DNA-binding residues of this peptide obliterate transfection activity. The introduction of a proline in the first turn of an α-helix of this 37-mer obliterates transfection activity, suggesting that the integrity of the α-helical structure of the N-terminal region of histone H2A is related to its transfection activity.

Bibliography

Balicki, D., Putnam, C. D., Scaria, P. V., & Beutler, E. (2002). Structure and function correlation in histone H2A peptide-mediated gene transfer. Proceedings of the National Academy of Sciences, 99(11), 7467â7471.

Authors 4

Danuta Balicki (first)
Christopher D. Putnam (additional)
Puthupparampil V. Scaria (additional)
Ernest Beutler (additional)

References 22 Referenced 64

10.1097/00005792-200201000-00005
10.1038/scientificamerican0697-102
10.1089/hum.1997.8.5-511
10.1007/978-1-4612-3490-6
10.1007/BF03401715
D Balicki Ph.D. thesis (The Scripps Research Institute, La Jolla, CA, 2001). / Ph.D. thesis by Balicki D (2001)
10.1089/hum.1996.7.12-1395
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I Demirhan, O Hasselmayer, A Chandra, M Ehemann, P Chandra J Hum Virol 1, 430–440 (1998). / J Hum Virol by Demirhan I (1998)
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10.1128/mcb.7.11.4048-4057.1987

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:46 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 7:38 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 12, 2024, 1:09 a.m.)
Issued	23 years, 3 months ago (May 28, 2002)
Published	23 years, 3 months ago (May 28, 2002)
Published Online	23 years, 3 months ago (May 28, 2002)
Published Print	23 years, 3 months ago (May 28, 2002)

Funders 0

None

BibTeX

@article{Balicki_2002, title={Structure and function correlation in histone H2A peptide-mediated gene transfer}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.102168299}, DOI={10.1073/pnas.102168299}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Balicki, Danuta and Putnam, Christopher D. and Scaria, Puthupparampil V. and Beutler, Ernest}, year={2002}, month=may, pages={7467–7471} }

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