Crystal structures of complexes containing domains from two viral internal ribosome entry site (IRES) RNAs bound to the 70S ribosome
10.1073/pnas.1018582108
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

Internal ribosome entry site (IRES) RNAs are elements of viral or cellular mRNAs that bypass steps of canonical eukaryotic cap-dependent translation initiation. Understanding of the structural basis of IRES mechanisms is limited, partially due to a lack of high-resolution structures of IRES RNAs bound to their cellular targets. Prompted by the universal phylogenetic conservation of the ribosomal P site, we solved the crystal structures of proposed P site binding domains from two intergenic region IRES RNAs bound to bacterial 70S ribosomes. The structures show that these IRES domains nearly perfectly mimic a tRNA•mRNA interaction. However, there are clear differences in the global shape and position of this IRES domain in the intersubunit space compared to those of tRNA, supporting a mechanism for IRES action that invokes hybrid state mimicry to drive a noncanonical mode of translocation. These structures suggest how relatively small structured RNAs can manipulate complex biological machines.

Bibliography

Zhu, J., Korostelev, A., Costantino, D. A., Donohue, J. P., Noller, H. F., & Kieft, J. S. (2011). Crystal structures of complexes containing domains from two viral internal ribosome entry site (IRES) RNAs bound to the 70S ribosome. Proceedings of the National Academy of Sciences, 108(5), 1839–1844.

Authors 6
  1. Jianyu Zhu (first)
  2. Andrei Korostelev (additional)
  3. David A. Costantino (additional)
  4. John P. Donohue (additional)
  5. Harry F. Noller (additional)
  6. Jeffrey S. Kieft (additional)
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Dates
Type When
Created 14 years, 7 months ago (Jan. 19, 2011, 12:04 a.m.)
Deposited 3 years, 2 months ago (June 7, 2022, 4:14 a.m.)
Indexed 2 months, 1 week ago (June 10, 2025, 5:30 a.m.)
Issued 14 years, 7 months ago (Jan. 18, 2011)
Published 14 years, 7 months ago (Jan. 18, 2011)
Published Online 14 years, 7 months ago (Jan. 18, 2011)
Published Print 14 years, 6 months ago (Feb. 1, 2011)
Funders 0

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@article{Zhu_2011, title={Crystal structures of complexes containing domains from two viral internal ribosome entry site (IRES) RNAs bound to the 70S ribosome}, volume={108}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1018582108}, DOI={10.1073/pnas.1018582108}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Zhu, Jianyu and Korostelev, Andrei and Costantino, David A. and Donohue, John P. and Noller, Harry F. and Kieft, Jeffrey S.}, year={2011}, month=jan, pages={1839–1844} }