DOI: 10.1073/pnas.1010005107. Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome

Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome

10.1073/pnas.1010005107

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant ( Triticum aestivum ) 80S ribosome at 5.5-Å resolution, together with a 6.1-Å map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92.5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribosome provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.

Bibliography

Armache, J.-P., Jarasch, A., Anger, A. M., Villa, E., Becker, T., Bhushan, S., Jossinet, F., Habeck, M., Dindar, G., Franckenberg, S., Marquez, V., Mielke, T., Thomm, M., Berninghausen, O., Beatrix, B., SÃ¶ding, J., Westhof, E., Wilson, D. N., & Beckmann, R. (2010). Localization of eukaryote-specific ribosomal proteins in a 5.5-â« cryo-EM map of the 80S eukaryotic ribosome. Proceedings of the National Academy of Sciences, 107(46), 19754â19759.

Authors 19

Jean-Paul Armache (first)
Alexander Jarasch (additional)
Andreas M. Anger (additional)
Elizabeth Villa (additional)
Thomas Becker (additional)
Shashi Bhushan (additional)
Fabrice Jossinet (additional)
Michael Habeck (additional)
Gülcin Dindar (additional)
Sibylle Franckenberg (additional)
Viter Marquez (additional)
Thorsten Mielke (additional)
Michael Thomm (additional)
Otto Berninghausen (additional)
Birgitta Beatrix (additional)
Johannes Söding (additional)
Eric Westhof (additional)
Daniel N. Wilson (additional)
Roland Beckmann (additional)

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Dates

Type	When
Created	14 years, 9 months ago (Oct. 26, 2010, 12:46 a.m.)
Deposited	3 years, 2 months ago (June 7, 2022, 3:18 a.m.)
Indexed	2 weeks, 2 days ago (Aug. 6, 2025, 9:39 a.m.)
Issued	14 years, 9 months ago (Oct. 25, 2010)
Published	14 years, 9 months ago (Oct. 25, 2010)
Published Online	14 years, 9 months ago (Oct. 25, 2010)
Published Print	14 years, 9 months ago (Nov. 16, 2010)

Funders 0

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BibTeX

@article{Armache_2010, title={Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome}, volume={107}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1010005107}, DOI={10.1073/pnas.1010005107}, number={46}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Armache, Jean-Paul and Jarasch, Alexander and Anger, Andreas M. and Villa, Elizabeth and Becker, Thomas and Bhushan, Shashi and Jossinet, Fabrice and Habeck, Michael and Dindar, Gülcin and Franckenberg, Sibylle and Marquez, Viter and Mielke, Thorsten and Thomm, Michael and Berninghausen, Otto and Beatrix, Birgitta and Söding, Johannes and Westhof, Eric and Wilson, Daniel N. and Beckmann, Roland}, year={2010}, month=oct, pages={19754–19759} }

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  "abstract": "<jats:p>\n            Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r\u00a0proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (\n            <jats:italic>Triticum aestivum</jats:italic>\n            ) 80S ribosome at 5.5-\u212b resolution, together with a 6.1-\u212b map of a translating\n            <jats:italic>Saccharomyces cerevisiae</jats:italic>\n            80S ribosome, we have localized and modeled 74/80 (92.5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribosome provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.\n          </jats:p>",
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