Structural basis for mRNA surveillance by archaeal Pelota and GTP-bound EF1α complex
10.1073/pnas.1009598107
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

No-go decay and nonstop decay are mRNA surveillance pathways that detect translational stalling and degrade the underlying mRNA, allowing the correct translation of the genetic code. In eukaryotes, the protein complex of Pelota (yeast Dom34) and Hbs1 translational GTPase recognizes the stalled ribosome containing the defective mRNA. Recently, we found that archaeal Pelota (aPelota) associates with archaeal elongation factor 1α (aEF1α) to act in the mRNA surveillance pathway, which accounts for the lack of an Hbs1 ortholog in archaea. Here we present the complex structure of aPelota and GTP-bound aEF1α determined at 2.3-Å resolution. The structure reveals how GTP-bound aEF1α recognizes aPelota and how aPelota in turn stabilizes the GTP form of aEF1α. Combined with the functional analysis in yeast, the present results provide structural insights into the molecular interaction between eukaryotic Pelota and Hbs1. Strikingly, the aPelota·aEF1α complex structurally resembles the tRNA·EF-Tu complex bound to the ribosome. Our findings suggest that the molecular mimicry of tRNA in the distorted “A/T state” conformation by Pelota enables the complex to efficiently detect and enter the empty A site of the stalled ribosome.

Bibliography

Kobayashi, K., Kikuno, I., Kuroha, K., Saito, K., Ito, K., Ishitani, R., Inada, T., & Nureki, O. (2010). Structural basis for mRNA surveillance by archaeal Pelota and GTP-bound EF1α complex. Proceedings of the National Academy of Sciences, 107(41), 17575–17579.

Authors 8
  1. Kan Kobayashi (first)
  2. Izumi Kikuno (additional)
  3. Kazushige Kuroha (additional)
  4. Kazuki Saito (additional)
  5. Koichi Ito (additional)
  6. Ryuichiro Ishitani (additional)
  7. Toshifumi Inada (additional)
  8. Osamu Nureki (additional)
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Dates
Type When
Created 14 years, 11 months ago (Sept. 28, 2010, 12:45 a.m.)
Deposited 3 years, 2 months ago (June 7, 2022, 3:12 a.m.)
Indexed 4 months ago (April 26, 2025, 2:21 a.m.)
Issued 14 years, 11 months ago (Sept. 27, 2010)
Published 14 years, 11 months ago (Sept. 27, 2010)
Published Online 14 years, 11 months ago (Sept. 27, 2010)
Published Print 14 years, 10 months ago (Oct. 12, 2010)
Funders 0

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@article{Kobayashi_2010, title={Structural basis for mRNA surveillance by archaeal Pelota and GTP-bound EF1α complex}, volume={107}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1009598107}, DOI={10.1073/pnas.1009598107}, number={41}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kobayashi, Kan and Kikuno, Izumi and Kuroha, Kazushige and Saito, Kazuki and Ito, Koichi and Ishitani, Ryuichiro and Inada, Toshifumi and Nureki, Osamu}, year={2010}, month=sep, pages={17575–17579} }