Microbisporicin gene cluster reveals unusual features of lantibiotic biosynthesis in actinomycetes
10.1073/pnas.1008285107
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

Lantibiotics are ribosomally synthesized, posttranslationally modified peptide antibiotics. The biosynthetic gene cluster for microbisporicin, a potent lantibiotic produced by the actinomycete Microbispora corallina containing chlorinated tryptophan and dihydroxyproline residues, was identified by genome scanning and isolated from an M. corallina cosmid library. Heterologous expression in Nonomuraea sp. ATCC 39727 confirmed that all of the genes required for microbisporicin biosynthesis were present in the cluster. Deletion, in M. corallina, of the gene ( mibA ) predicted to encode the prepropeptide abolished microbisporicin production. Further deletion analysis revealed insights into the biosynthesis of this unusual and potentially clinically useful lantibiotic, shedding light on mechanisms of regulation and self-resistance. In particular, we report an example of the involvement of a tryptophan halogenase in the modification of a ribosomally synthesized peptide and the pathway-specific regulation of an antibiotic biosynthetic gene cluster by an extracytoplasmic function σ factor–anti-σ factor complex.

Bibliography

Foulston, L. C., & Bibb, M. J. (2010). Microbisporicin gene cluster reveals unusual features of lantibiotic biosynthesis in actinomycetes. Proceedings of the National Academy of Sciences, 107(30), 13461–13466.

Authors 2
  1. Lucy C. Foulston (first)
  2. Mervyn J. Bibb (additional)
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Dates
Type When
Created 15 years, 1 month ago (July 13, 2010, 9:46 p.m.)
Deposited 3 years, 2 months ago (June 7, 2022, 2:58 a.m.)
Indexed 3 months ago (June 3, 2025, 11:15 a.m.)
Issued 15 years, 1 month ago (July 13, 2010)
Published 15 years, 1 month ago (July 13, 2010)
Published Online 15 years, 1 month ago (July 13, 2010)
Published Print 15 years, 1 month ago (July 27, 2010)
Funders 0

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@article{Foulston_2010, title={Microbisporicin gene cluster reveals unusual features of lantibiotic biosynthesis in actinomycetes}, volume={107}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1008285107}, DOI={10.1073/pnas.1008285107}, number={30}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Foulston, Lucy C. and Bibb, Mervyn J.}, year={2010}, month=jul, pages={13461–13466} }