DOI: 10.1073/pnas.1007835107. Dynamics of podosome stiffness revealed by atomic force microscopy

Dynamics of podosome stiffness revealed by atomic force microscopy

10.1073/pnas.1007835107

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Podosomes are unique cellular entities specifically found in macrophages and involved in cell–matrix interactions, matrix degradation, and 3D migration. They correspond to a core of F-actin surrounded at its base by matrix receptors. To investigate the structure/function relationships of podosomes, soft lithography, atomic force microscopy (AFM), and correlative fluorescence microscopy were used to characterize podosome physical properties in macrophages differentiated from human blood monocytes. Podosome formation was restricted to delineated areas with micropatterned fibrinogen to facilitate AFM analyses. Podosome height and stiffness were measured with great accuracy in living macrophages (578 ± 209 nm and 43.8 ± 9.3 kPa) and these physical properties were independent of the nature of the underlying matrix. In addition, time-lapse AFM revealed that podosomes harbor two types of overlapping periodic stiffness variations throughout their lifespan, which depend on F-actin and myosin II activity. This report shows that podosome biophysical properties are amenable to AFM, allowing the study of podosomes in living macrophages at nanoscale resolution and the analysis of their intimate dynamics. Such an approach opens up perspectives to better understand the mechanical functionality of podosomes under physiological and pathological contexts.

Bibliography

Labernadie, A., Thibault, C., Vieu, C., Maridonneau-Parini, I., & CharriÃ¨re, G. M. (2010). Dynamics of podosome stiffness revealed by atomic force microscopy. Proceedings of the National Academy of Sciences, 107(49), 21016â21021.

Authors 5

Anna Labernadie (first)
Christophe Thibault (additional)
Christophe Vieu (additional)
Isabelle Maridonneau-Parini (additional)
Guillaume M. Charrière (additional)

References 31 Referenced 140

10.1182/blood-2009-04-218735
E Van Goethem, et al., Macrophage podosomes go 3D. Eur J Cell Biol, in press. (2010). / Eur J Cell Biol / Macrophage podosomes go 3D by Van Goethem E (2010)
10.1242/jcs.032631
10.1371/journal.pone.0000179
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10.1242/jcs.052704
10.1529/biophysj.108.133462
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H Hertz, Über die Berührung fester elastischer Körper. J Reine Angewandte Mathematik 92, 156–171, (in German). (1882). (10.1515/crll.1882.92.156) / J Reine Angewandte Mathematik / Über die Berührung fester elastischer Körper by Hertz H (1882)
10.1016/0020-7225(65)90019-4

Dates

Type	When
Created	14 years, 9 months ago (Nov. 16, 2010, 10:38 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 8:03 p.m.)
Indexed	1 month, 1 week ago (July 24, 2025, 7:14 a.m.)
Issued	14 years, 9 months ago (Nov. 16, 2010)
Published	14 years, 9 months ago (Nov. 16, 2010)
Published Online	14 years, 9 months ago (Nov. 16, 2010)
Published Print	14 years, 8 months ago (Dec. 7, 2010)

Funders 0

None

BibTeX

@article{Labernadie_2010, title={Dynamics of podosome stiffness revealed by atomic force microscopy}, volume={107}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1007835107}, DOI={10.1073/pnas.1007835107}, number={49}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Labernadie, Anna and Thibault, Christophe and Vieu, Christophe and Maridonneau-Parini, Isabelle and Charrière, Guillaume M.}, year={2010}, month=nov, pages={21016–21021} }

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