Abstract
Adding reagents to drops is one of the most important functions in droplet-based microfluidic systems; however, a robust technique to accomplish this does not exist. Here, we introduce the picoinjector, a robust device to add controlled volumes of reagent using electro-microfluidics at kilohertz rates. It can also perform multiple injections for serial and combinatorial additions.
References
25
Referenced
454
10.1073/pnas.47.12.1981
10.1002/anie.200601554
10.1103/PhysRevLett.86.4163
10.1063/1.1537519
10.1063/1.2164911
10.1073/pnas.0910781107
10.1073/pnas.0708321105
10.1016/j.chembiol.2008.08.004
10.1039/b802941e
-
A Huebner et al. Quantitative detection of protein expression in single cells using droplet microfluidics. Chem Commun 12 1218–1220 (2007).
(
10.1039/b618570c
) 10.1002/anie.200602946
10.1021/ac0601718
10.1063/1.2218058
10.1103/PhysRevLett.83.2359
10.1063/1.2357039
10.1007/BF02477781
10.1039/B815002H
10.1016/j.cbpa.2006.04.004
10.1073/pnas.0607502103
10.1038/nrg1696
10.1038/nrg1618
10.1038/nbt.1583
10.1021/ac980656z
10.1002/adma.200601787
10.1039/b806706f
Dates
Type | When |
---|---|
Created | 14 years, 10 months ago (Oct. 20, 2010, 11:15 p.m.) |
Deposited | 3 years, 4 months ago (April 12, 2022, 7:06 p.m.) |
Indexed | 5 days, 3 hours ago (Aug. 27, 2025, 11:47 a.m.) |
Issued | 14 years, 10 months ago (Oct. 20, 2010) |
Published | 14 years, 10 months ago (Oct. 20, 2010) |
Published Online | 14 years, 10 months ago (Oct. 20, 2010) |
Published Print | 14 years, 9 months ago (Nov. 9, 2010) |
@article{Abate_2010, title={High-throughput injection with microfluidics using picoinjectors}, volume={107}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1006888107}, DOI={10.1073/pnas.1006888107}, number={45}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Abate, Adam R. and Hung, Tony and Mary, Pascaline and Agresti, Jeremy J. and Weitz, David A.}, year={2010}, month=oct, pages={19163–19166} }