DOI: 10.1073/pnas.1005949107. Non-Mendelian determinant [ <i>ISP</i> <sup>+</sup> ] in yeast is a nuclear-residing prion form of the global transcriptional regulator Sfp1

Non-Mendelian determinant [ ISP + ] in yeast is a nuclear-residing prion form of the global transcriptional regulator Sfp1

10.1073/pnas.1005949107

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Four protein-based genetic determinants or prions—[ SWI + ], [ MCA ], [ OCT + ], and [ MOT3 + ]—are recent additions to the list of well-known Saccharomyces cerevisiae prions, [ PSI + ], [ URE3 ], and [ PIN + ]. A rapid expansion of this list may indicate that many yeast proteins can convert into heritable prion forms and underscores a problem of prion input into cellular physiology. Here, we prove that the global transcriptional regulator Sfp1 can become a prion corresponding to the prion-like determinant [ ISP + ] described earlier. We show that SFP1 deletion causes an irreversible [ ISP + ] loss, whereas increased SFP1 expression induces [ ISP + ] appearance. Cells that display the [ ISP + ] phenotype contain the aggregated form of Sfp1. Indeed, these aggregates demonstrate a nuclear location. We also show that the phenotypic manifestation of Sfp1 prionization differs from the manifestation of SFP1 deletion. These properties and others distinguish [ ISP + ] from yeast prions described to date.

Bibliography

Rogoza, T., Goginashvili, A., Rodionova, S., Ivanov, M., Viktorovskaya, O., Rubel, A., Volkov, K., & Mironova, L. (2010). Non-Mendelian determinant [ ISP + ] in yeast is a nuclear-residing prion form of the global transcriptional regulator Sfp1. Proceedings of the National Academy of Sciences, 107(23), 10573â10577.

Authors 8

Tatyana Rogoza (first)
Alexander Goginashvili (additional)
Sofia Rodionova (additional)
Maxim Ivanov (additional)
Olga Viktorovskaya (additional)
Alexander Rubel (additional)
Kirill Volkov (additional)
Ludmila Mironova (additional)

References 39 Referenced 105

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Dates

Type	When
Created	15 years, 3 months ago (May 24, 2010, 11:59 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 6:25 p.m.)
Indexed	37 minutes ago (Sept. 3, 2025, 6:20 a.m.)
Issued	15 years, 3 months ago (May 24, 2010)
Published	15 years, 3 months ago (May 24, 2010)
Published Online	15 years, 3 months ago (May 24, 2010)
Published Print	15 years, 2 months ago (June 8, 2010)

Funders 0

None

BibTeX

@article{Rogoza_2010, title={Non-Mendelian determinant [ ISP + ] in yeast is a nuclear-residing prion form of the global transcriptional regulator Sfp1}, volume={107}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1005949107}, DOI={10.1073/pnas.1005949107}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Rogoza, Tatyana and Goginashvili, Alexander and Rodionova, Sofia and Ivanov, Maxim and Viktorovskaya, Olga and Rubel, Alexander and Volkov, Kirill and Mironova, Ludmila}, year={2010}, month=may, pages={10573–10577} }

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