DOI: 10.1073/pnas.1002123107. Structural organization of a filamentous influenza A virus

Structural organization of a filamentous influenza A virus

10.1073/pnas.1002123107

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Influenza is a lipid-enveloped, pleomorphic virus. We combine electron cryotomography and analysis of images of frozen-hydrated virions to determine the structural organization of filamentous influenza A virus. Influenza A/Udorn/72 virions are capsule-shaped or filamentous particles of highly uniform diameter. We show that the matrix layer adjacent to the membrane is an ordered helix of the M1 protein and its close interaction with the surrounding envelope determines virion morphology. The ribonucleoprotein particles (RNPs) that package the genome segments form a tapered assembly at one end of the virus interior. The neuraminidase, which is present in smaller numbers than the hemagglutinin, clusters in patches and are typically present at the end of the virion opposite to RNP attachment. Incubation of virus at low pH causes a loss of filamentous morphology, during which we observe a structural transition of the matrix layer from its helical, membrane-associated form to a multilayered coil structure inside the virus particle. The polar organization of the virus provides a model for assembly of the virion during budding at the host membrane. Images and tomograms of A/Aichi/68 X-31 virions show the generality of these conclusions to non-filamentous virions.

Bibliography

Calder, L. J., Wasilewski, S., Berriman, J. A., & Rosenthal, P. B. (2010). Structural organization of a filamentous influenza A virus. Proceedings of the National Academy of Sciences, 107(23), 10685â10690.

Authors 4

Lesley J. Calder (first)
Sebastian Wasilewski (additional)
John A. Berriman (additional)
Peter B. Rosenthal (additional)

References 50 Referenced 213

Dates

Type	When
Created	15 years, 3 months ago (May 24, 2010, 11:59 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 6:28 p.m.)
Indexed	3 weeks, 4 days ago (Aug. 3, 2025, 12:20 a.m.)
Issued	15 years, 3 months ago (May 24, 2010)
Published	15 years, 3 months ago (May 24, 2010)
Published Online	15 years, 3 months ago (May 24, 2010)
Published Print	15 years, 2 months ago (June 8, 2010)

Funders 0

None

BibTeX

@article{Calder_2010, title={Structural organization of a filamentous influenza A virus}, volume={107}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1002123107}, DOI={10.1073/pnas.1002123107}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Calder, Lesley J. and Wasilewski, Sebastian and Berriman, John A. and Rosenthal, Peter B.}, year={2010}, month=may, pages={10685–10690} }

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