DOI: 10.1073/pnas.092152799. Modulation of tRNA <sup>Ala</sup> identity by inorganic pyrophosphatase

Modulation of tRNA Ala identity by inorganic pyrophosphatase

10.1073/pnas.092152799

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A highly sensitive assay of tRNA aminoacylation was developed that directly measures the fraction of aminoacylated tRNA by following amino acid attachment to the 3′- 32 P-labeled tRNA. When applied to Escherichia coli alanyl-tRNA synthetase, the assay allowed accurate measurement of aminoacylation of the most deleterious mutants of tRNA Ala . The effect of tRNA Ala identity mutations on both aminoacylation efficiency ( k cat / K M ) and steady-state level of aminoacyl-tRNA was evaluated in the absence and presence of inorganic pyrophosphatase and elongation factor Tu. Significant levels of aminoacylation were achieved for tRNA mutants even when the k cat / K M value is reduced by as much as several thousandfold. These results partially reconcile the discrepancy between in vivo and in vitro analysis of tRNA Ala identity.

Bibliography

Wolfson, A. D., & Uhlenbeck, O. C. (2002). Modulation of tRNA Ala identity by inorganic pyrophosphatase. Proceedings of the National Academy of Sciences, 99(9), 5965â5970.

Authors 2

Alexey D. Wolfson (first)
Olke C. Uhlenbeck (additional)

References 51 Referenced 108

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:46 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 7:22 p.m.)
Indexed	1 month, 3 weeks ago (July 4, 2025, 6:43 a.m.)
Issued	23 years, 3 months ago (April 30, 2002)
Published	23 years, 3 months ago (April 30, 2002)
Published Online	23 years, 3 months ago (April 30, 2002)
Published Print	23 years, 3 months ago (April 30, 2002)

Funders 0

None

BibTeX

@article{Wolfson_2002, title={Modulation of tRNA Ala identity by inorganic pyrophosphatase}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.092152799}, DOI={10.1073/pnas.092152799}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wolfson, Alexey D. and Uhlenbeck, Olke C.}, year={2002}, month=apr, pages={5965–5970} }

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