DOI: 10.1073/pnas.0914218107. Mapping amorphous calcium phosphate transformation into crystalline mineral from the cell to the bone in zebrafish fin rays

Mapping amorphous calcium phosphate transformation into crystalline mineral from the cell to the bone in zebrafish fin rays

10.1073/pnas.0914218107

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The continuously forming fin bony rays of zebrafish represent a simple bone model system in which mineralization is temporally and spatially resolved. The mineralized collagen fibrils of the fin bones are identical in structure to those found in all known bone materials. We study the continuous mineralization process within the tissue by using synchrotron microbeam x-ray diffraction and small-angle scattering, combined with cryo-scanning electron microscopy. The former provides information on the mineral phase and the mineral particles size and shape, whereas the latter allows high-resolution imaging of native hydrated tissues. The integration of the two techniques demonstrates that new mineral is delivered and deposited as packages of amorphous calcium phosphate nanospheres, which transform into platelets of crystalline apatite within the collagen matrix.

Bibliography

Mahamid, J., Aichmayer, B., Shimoni, E., Ziblat, R., Li, C., Siegel, S., Paris, O., Fratzl, P., Weiner, S., & Addadi, L. (2010). Mapping amorphous calcium phosphate transformation into crystalline mineral from the cell to the bone in zebrafish fin rays. Proceedings of the National Academy of Sciences, 107(14), 6316â6321.

Authors 10

Julia Mahamid (first)
Barbara Aichmayer (additional)
Eyal Shimoni (additional)
Roy Ziblat (additional)
Chenghao Li (additional)
Stefan Siegel (additional)
Oskar Paris (additional)
Peter Fratzl (additional)
Steve Weiner (additional)
Lia Addadi (additional)

References 33 Referenced 402

10.1016/j.bone.2006.02.058
10.1016/j.bone.2007.04.176
10.1016/j.mser.2007.05.001
10.1016/0022-0248(89)90102-4
10.1016/j.bone.2006.02.059
10.1073/pnas.0803354105
10.1016/j.jsb.2009.02.001
10.1021/cg800252f
10.1007/s11706-009-0036-x
10.1116/1.2955443
10.1529/biophysj.107.128355
10.1002/macp.200400066
10.1016/j.msec.2006.01.006
10.1016/j.msec.2005.06.063
10.1002/ar.1092280404
10.1007/BF00216033
10.1007/BF00496739
10.1002/(SICI)1097-4687(199903)239:3<297::AID-JMOR6>3.0.CO;2-U
10.1016/S0044-8486(02)00135-7
P Fratzl, et al., Diffracting “stacks of cards”—Some thoughts about small-angle scattering from bone. Prog Coll Pol Sci S 130, 33–39 (2005). / Prog Coll Pol Sci S / Diffracting “stacks of cards”—Some thoughts about small-angle scattering from bone by Fratzl P (2005)
10.1007/BF02556454
P Fratzl, S Schreiber, K Klaushofer, Bone mineralization as studied by small-angle x-ray scattering. Connect Tissue Res 35, 9–16 (1996). / Connect Tissue Res / Bone mineralization as studied by small-angle x-ray scattering by Fratzl P (1996)
A Guinier, G Fournet Small-Angle Scattering of X-Rays (Wiley, New York, 1955). / Small-Angle Scattering of X-Rays by Guinier A (1955)
10.1007/BF02563989
10.1023/A:1026425404169
10.1002/adma.200300381
10.1074/jbc.M210868200
10.1111/j.1742-4658.2009.06958.x
10.1074/jbc.M109.020370
10.1371/journal.pone.0005634
10.1107/S0021889806045444
AP Hammersley FIT2D: An Introduction and Overview (European Synchrotron Radiation Facility Grenoble internal report. (1997).
10.1111/j.1365-2818.2007.01732.x

Dates

Type	When
Created	15 years, 4 months ago (March 22, 2010, 10:36 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 7:22 p.m.)
Indexed	2 weeks, 6 days ago (Aug. 2, 2025, 1:20 a.m.)
Issued	15 years, 5 months ago (March 22, 2010)
Published	15 years, 5 months ago (March 22, 2010)
Published Online	15 years, 5 months ago (March 22, 2010)
Published Print	15 years, 4 months ago (April 6, 2010)

Funders 0

None

BibTeX

@article{Mahamid_2010, title={Mapping amorphous calcium phosphate transformation into crystalline mineral from the cell to the bone in zebrafish fin rays}, volume={107}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0914218107}, DOI={10.1073/pnas.0914218107}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Mahamid, Julia and Aichmayer, Barbara and Shimoni, Eyal and Ziblat, Roy and Li, Chenghao and Siegel, Stefan and Paris, Oskar and Fratzl, Peter and Weiner, Steve and Addadi, Lia}, year={2010}, month=mar, pages={6316–6321} }

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