DOI: 10.1073/pnas.0913730107. Two competing orientation patterns explain experimentally observed anomalies in growing actin networks

Two competing orientation patterns explain experimentally observed anomalies in growing actin networks

10.1073/pnas.0913730107

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The lamellipodium of migrating animal cells protrudes by directed polymerization of a branched actin network. The underlying mechanisms of filament growth, branching, and capping can be studied in in vitro assays. However, conflicting results have been reported for the force–velocity relation of such actin networks, namely both convex and concave shapes as well as history dependencies. Here we model branching as a reaction that is independent of the number of existing filaments, in contrast to capping, which is assumed to be proportional to the number of existing filaments. Using both stochastic network simulations and deterministic rate equations, we show that such a description naturally leads to the stability of two qualitatively different stationary states of the system, namely a ± 35° and a +70/0/-70° orientation pattern. Changes in network growth velocity induce a transition between these two patterns. For sufficiently different protrusion efficiency of the two network architectures, this leads to hysteresis in the growth velocity of actin networks under force. Dependent on the history of the system, convex and concave regimes are obtained for the force–velocity relation. Thus a simple generic model can explain the experimentally observed anomalies, with far reaching consequences for cell migration.

Authors 2

Julian Weichsel (first)
Ulrich S. Schwarz (additional)

References 29 Referenced 71

Dates

Type	When
Created	15 years, 5 months ago (March 22, 2010, 10:36 p.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 7:26 p.m.)
Indexed	1 month, 2 weeks ago (July 8, 2025, 3:45 a.m.)
Issued	15 years, 5 months ago (March 22, 2010)
Published	15 years, 5 months ago (March 22, 2010)
Published Online	15 years, 5 months ago (March 22, 2010)
Published Print	15 years, 4 months ago (April 6, 2010)

Funders 0

None

BibTeX

@article{Weichsel_2010, title={Two competing orientation patterns explain experimentally observed anomalies in growing actin networks}, volume={107}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0913730107}, DOI={10.1073/pnas.0913730107}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Weichsel, Julian and Schwarz, Ulrich S.}, year={2010}, month=mar, pages={6304–6309} }

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