DOI: 10.1073/pnas.0909207107. Evidence of an epithelial stem/progenitor cell hierarchy in the adult mouse lung

Evidence of an epithelial stem/progenitor cell hierarchy in the adult mouse lung

10.1073/pnas.0909207107

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The role of lung epithelial stem cells in maintenance and repair of the adult lung is ill-defined, and their identity remains contentious because of the lack of definitive markers for their prospective isolation and the absence of clonogenic assays able to measure their stem/progenitor cell potential. In this study, we show that replication of epithelial–mesenchymal interactions in a previously undescribed matrigel-based clonogenic assay enables the identification of lung epithelial stem/progenitor cells by their colony-forming potential in vitro. We describe a population of EpCAM hi CD49f pos CD104 pos CD24 low epithelial cfus that generate colonies comprising airway, alveolar, or mixed lung epithelial cell lineages when cocultured with EpCAM neg Sca-1 pos lung mesenchymal cells. We show that soluble fibroblast growth factor-10 and hepatocyte growth factor partially replace the requirement for mesenchymal support of epithelial colony formation, allowing clonal passaging and demonstration of their capacity for self-renewal. These data support a model in which the adult mouse lung contains a minor population of multipotent epithelial stem/progenitor cells with the capacity for self-renewal and whose descendants give rise to airway and alveolar epithelial cell lineages in vitro.

Authors 4

Jonathan L. McQualter (first)
Karen Yuen (additional)
Brenda Williams (additional)
Ivan Bertoncello (additional)

References 22 Referenced 349

Dates

Type	When
Created	15 years, 7 months ago (Jan. 4, 2010, 9:36 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 6:50 p.m.)
Indexed	1 day, 18 hours ago (Aug. 27, 2025, 11:45 a.m.)
Issued	15 years, 7 months ago (Jan. 4, 2010)
Published	15 years, 7 months ago (Jan. 4, 2010)
Published Online	15 years, 7 months ago (Jan. 4, 2010)
Published Print	15 years, 7 months ago (Jan. 26, 2010)

Funders 0

None

BibTeX

@article{McQualter_2010, title={Evidence of an epithelial stem/progenitor cell hierarchy in the adult mouse lung}, volume={107}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0909207107}, DOI={10.1073/pnas.0909207107}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={McQualter, Jonathan L. and Yuen, Karen and Williams, Brenda and Bertoncello, Ivan}, year={2010}, month=jan, pages={1414–1419} }

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