DOI: 10.1073/pnas.0905966106. The 3-dimensional structure of a hepatitis C virus p7 ion channel by electron microscopy

The 3-dimensional structure of a hepatitis C virus p7 ion channel by electron microscopy

10.1073/pnas.0905966106

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Infection with the hepatitis C virus (HCV) has a huge impact on global health putting more than 170 million people at risk of developing severe liver disease. The HCV encoded p7 ion channel is essential for the production of infectious viruses. Despite a growing body of functional data, little is known about the 3-dimensional (3D) structure of the channel. Here, we present the 3D structure of a full-length viroporin, the detergent-solubilized hexameric 42 kDa form of the HCV p7 ion channel, as determined by single-particle electron microscopy using the random conical tilting approach. The reconstruction of such a small protein complex was made possible by a combination of high-contrast staining, the symmetry, and the distinct structural features of the channel. The orientation of the p7 monomers within the density was established using immunolabeling with N and C termini specific F ab fragments. The density map at a resolution of ≈16 Å reveals a flower-shaped protein architecture with protruding petals oriented toward the ER lumen. This broadest part of the channel presents a comparatively large surface area providing potential interaction sites for cellular and virally encoded ER resident proteins.

Bibliography

Luik, P., Chew, C., Aittoniemi, J., Chang, J., Wentworth, P., Dwek, R. A., Biggin, P. C., VÃ©nien-Bryan, C., & Zitzmann, N. (2009). The 3-dimensional structure of a hepatitis C virus p7 ion channel by electron microscopy. Proceedings of the National Academy of Sciences, 106(31), 12712â12716.

Authors 9

Philipp Luik (first)
Chee Chew (additional)
Jussi Aittoniemi (additional)
Jason Chang (additional)
Paul Wentworth (additional)
Raymond A. Dwek (additional)
Philip C. Biggin (additional)
Catherine Vénien-Bryan (additional)
Nicole Zitzmann (additional)

References 36 Referenced 132

Dates

Type	When
Created	16 years, 1 month ago (July 9, 2009, 9:45 p.m.)
Deposited	3 years, 2 months ago (May 23, 2022, 4:59 p.m.)
Indexed	2 months ago (June 17, 2025, 11:30 a.m.)
Issued	16 years ago (Aug. 4, 2009)
Published	16 years ago (Aug. 4, 2009)
Published Online	16 years ago (Aug. 4, 2009)
Published Print	16 years ago (Aug. 4, 2009)

Funders 0

None

BibTeX

@article{Luik_2009, title={The 3-dimensional structure of a hepatitis C virus p7 ion channel by electron microscopy}, volume={106}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0905966106}, DOI={10.1073/pnas.0905966106}, number={31}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Luik, Philipp and Chew, Chee and Aittoniemi, Jussi and Chang, Jason and Wentworth, Paul and Dwek, Raymond A. and Biggin, Philip C. and Vénien-Bryan, Catherine and Zitzmann, Nicole}, year={2009}, month=aug, pages={12712–12716} }

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