DOI: 10.1073/pnas.0905819106. Postsynaptic Neuroligin1 regulates presynaptic maturation

Postsynaptic Neuroligin1 regulates presynaptic maturation

10.1073/pnas.0905819106

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Presynaptic nerve terminals pass through distinct stages of maturation after their initial assembly. Here we show that the postsynaptic cell adhesion molecule Neuroligin1 regulates key steps of presynaptic maturation. Presynaptic terminals from Neuroligin1-knockout mice remain structurally and functionally immature with respect to active zone stability and synaptic vesicle pool size, as analyzed in cultured hippocampal neurons. Conversely, overexpression of Neuroligin1 in immature neurons, that is within the first 5 days after plating, induced the formation of presynaptic boutons that had hallmarks of mature boutons. In particular, Neuroligin1 enhanced the size of the pool of recycling synaptic vesicles, the rate of synaptic vesicle exocytosis, the fraction of boutons responding to depolarization, as well as the responsiveness of the presynaptic release machinery to phorbol ester stimulation. Moreover, Neuroligin1 induced the formation of active zones that remained stable in the absence of F-actin, another hallmark of advanced maturation. Acquisition of F-actin independence of the active zone marker Bassoon during culture development or induced via overexpression of Neuroligin1 was activity-dependent. The extracellular domain of Neuroligin1 was sufficient to induce assembly of functional presynaptic terminals, while the intracellular domain was required for terminal maturation. These data show that induction of presynaptic terminal assembly and maturation involve mechanistically distinct actions of Neuroligins, and that Neuroligin1 is essential for presynaptic terminal maturation.

Bibliography

Wittenmayer, N., KÃ¶rber, C., Liu, H., Kremer, T., Varoqueaux, F., Chapman, E. R., Brose, N., Kuner, T., & Dresbach, T. (2009). Postsynaptic Neuroligin1 regulates presynaptic maturation. Proceedings of the National Academy of Sciences, 106(32), 13564â13569.

Authors 9

Nina Wittenmayer (first)
Christoph Körber (additional)
Huisheng Liu (additional)
Thomas Kremer (additional)
Frederique Varoqueaux (additional)
Edwin R. Chapman (additional)
Nils Brose (additional)
Thomas Kuner (additional)
Thomas Dresbach (additional)

References 30 Referenced 84

Dates

Type	When
Created	16 years, 1 month ago (July 23, 2009, 9:36 p.m.)
Deposited	3 years, 3 months ago (May 23, 2022, 5 p.m.)
Indexed	3 months, 1 week ago (May 20, 2025, 12:48 p.m.)
Issued	16 years ago (Aug. 11, 2009)
Published	16 years ago (Aug. 11, 2009)
Published Online	16 years ago (Aug. 11, 2009)
Published Print	16 years ago (Aug. 11, 2009)

Funders 0

None

BibTeX

@article{Wittenmayer_2009, title={Postsynaptic Neuroligin1 regulates presynaptic maturation}, volume={106}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0905819106}, DOI={10.1073/pnas.0905819106}, number={32}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wittenmayer, Nina and Körber, Christoph and Liu, Huisheng and Kremer, Thomas and Varoqueaux, Frederique and Chapman, Edwin R. and Brose, Nils and Kuner, Thomas and Dresbach, Thomas}, year={2009}, month=aug, pages={13564–13569} }

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