DOI: 10.1073/pnas.0905007106. Comparison of Alzheimer Aβ(1–40) and Aβ(1–42) amyloid fibrils reveals similar protofilament structures

Comparison of Alzheimer Aβ(1–40) and Aβ(1–42) amyloid fibrils reveals similar protofilament structures

10.1073/pnas.0905007106

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We performed mass-per-length (MPL) measurements and electron cryomicroscopy (cryo-EM) with 3D reconstruction on an Aβ(1–42) amyloid fibril morphology formed under physiological pH conditions. The data show that the examined Aβ(1–42) fibril morphology has only one protofilament, although two protofilaments were observed with a previously studied Aβ(1–40) fibril. The latter fibril was resolved at 8 Å resolution showing pairs of β-sheets at the cores of the two protofilaments making up a fibril. Detailed comparison of the Aβ(1–42) and Aβ(1–40) fibril structures reveals that they share an axial twofold symmetry and a similar protofilament structure. Furthermore, the MPL data indicate that the protofilaments of the examined Aβ(1–40) and Aβ(1–42) fibrils have the same number of Aβ molecules per cross-β repeat. Based on this data and the previously studied Aβ(1–40) fibril structure, we describe a model for the arrangement of peptides within the Aβ(1–42) fibril.

Bibliography

Schmidt, M., Sachse, C., Richter, W., Xu, C., FÃ¤ndrich, M., & Grigorieff, N. (2009). Comparison of Alzheimer AÎ²(1â40) and AÎ²(1â42) amyloid fibrils reveals similar protofilament structures. Proceedings of the National Academy of Sciences, 106(47), 19813â19818.

Authors 6

Matthias Schmidt (first)
Carsten Sachse (additional)
Walter Richter (additional)
Chen Xu (additional)
Marcus Fändrich (additional)
Nikolaus Grigorieff (additional)

References 38 Referenced 245

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Dates

Type	When
Created	15 years, 10 months ago (Oct. 20, 2009, 9:50 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 5:11 p.m.)
Indexed	1 minute ago (Sept. 2, 2025, 12:37 p.m.)
Issued	15 years, 9 months ago (Nov. 24, 2009)
Published	15 years, 9 months ago (Nov. 24, 2009)
Published Online	15 years, 9 months ago (Nov. 24, 2009)
Published Print	15 years, 9 months ago (Nov. 24, 2009)

Funders 0

None

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