DOI: 10.1073/pnas.0904565106. Quantifying cellular traction forces in three dimensions

Quantifying cellular traction forces in three dimensions

10.1073/pnas.0904565106

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Cells engage in mechanical force exchange with their extracellular environment through tension generated by the cytoskeleton. A method combining laser scanning confocal microscopy (LSCM) and digital volume correlation (DVC) enables tracking and quantification of cell-mediated deformation of the extracellular matrix in all three spatial dimensions. Time-lapse confocal imaging of migrating 3T3 fibroblasts on fibronectin (FN)-modified polyacrylamide gels of varying thickness reveals significant in-plane (x, y) and normal (z) displacements, and illustrates the extent to which cells, even in nominally two-dimensional (2-D) environments, explore their surroundings in all three dimensions. The magnitudes of the measured displacements are independent of the elastic moduli of the gels. Analysis of the normal displacement profiles suggests that normal forces play important roles even in 2-D cell migration.

Bibliography

Maskarinec, S. A., Franck, C., Tirrell, D. A., & Ravichandran, G. (2009). Quantifying cellular traction forces in three dimensions. Proceedings of the National Academy of Sciences, 106(52), 22108â22113.

Authors 4

Stacey A. Maskarinec (first)
Christian Franck (additional)
David A. Tirrell (additional)
Guruswami Ravichandran (additional)

References 29 Referenced 260

Dates

Type	When
Created	15 years, 8 months ago (Dec. 15, 2009, 11:19 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 5:10 p.m.)
Indexed	1 week, 2 days ago (Aug. 23, 2025, 9:45 p.m.)
Issued	15 years, 8 months ago (Dec. 29, 2009)
Published	15 years, 8 months ago (Dec. 29, 2009)
Published Online	15 years, 8 months ago (Dec. 29, 2009)
Published Print	15 years, 8 months ago (Dec. 29, 2009)

Funders 0

None

BibTeX

@article{Maskarinec_2009, title={Quantifying cellular traction forces in three dimensions}, volume={106}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0904565106}, DOI={10.1073/pnas.0904565106}, number={52}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Maskarinec, Stacey A. and Franck, Christian and Tirrell, David A. and Ravichandran, Guruswami}, year={2009}, month=dec, pages={22108–22113} }

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