DOI: 10.1073/pnas.0902752106. Flexible electrical recording from cells using nanowire transistor arrays

Flexible electrical recording from cells using nanowire transistor arrays

10.1073/pnas.0902752106

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Semiconductor nanowires (NWs) have unique electronic properties and sizes comparable with biological structures involved in cellular communication, thus making them promising nanostructures for establishing active interfaces with biological systems. We report a flexible approach to interface NW field-effect transistors (NWFETs) with cells and demonstrate this for silicon NWFET arrays coupled to embryonic chicken cardiomyocytes. Cardiomyocyte cells were cultured on thin, optically transparent polydimethylsiloxane (PDMS) sheets and then brought into contact with Si-NWFET arrays fabricated on standard substrates. NWFET conductance signals recorded from cardiomyocytes exhibited excellent signal-to-noise ratios with values routinely >5 and signal amplitudes that were tuned by varying device sensitivity through changes in water gate–voltage potential, V g . Signals recorded from cardiomyocytes for V g from −0.5 to +0.1 V exhibited amplitude variations from 31 to 7 nS whereas the calibrated voltage remained constant, indicating a robust NWFET/cell interface. In addition, signals recorded as a function of increasing/decreasing displacement of the PDMS/cell support to the device chip showed a reversible >2× increase in signal amplitude (calibrated voltage) from 31 nS (1.0 mV) to 72 nS (2.3 mV). Studies with the displacement close to but below the point of cell disruption yielded calibrated signal amplitudes as large as 10.5 ± 0.2 mV. Last, multiplexed recording of signals from NWFET arrays interfaced to cardiomyocyte monolayers enabled temporal shifts and signal propagation to be determined with good spatial and temporal resolution. Our modular approach simplifies the process of interfacing cardiomyocytes and other cells to high-performance Si-NWFETs, thus increasing the experimental versatility of NWFET arrays and enabling device registration at the subcellular level.

Bibliography

Cohen-Karni, T., Timko, B. P., Weiss, L. E., & Lieber, C. M. (2009). Flexible electrical recording from cells using nanowire transistor arrays. Proceedings of the National Academy of Sciences, 106(18), 7309â7313.

Authors 4

Tzahi Cohen-Karni (first)
Brian P. Timko (additional)
Lucien E. Weiss (additional)
Charles M. Lieber (additional)

References 33 Referenced 201

B Hille Ion Channels of Excitable Membranes (Sinauer, Sunderland, MA, 2001). / Ion Channels of Excitable Membranes by Hille B (2001)
DP Zipes, J Jalife Cardiac Electrophysiology : From Cell to Bedside (Saunders, Philadelphia, 2004). / Cardiac Electrophysiology : From Cell to Bedside by Zipes DP (2004)
10.1007/b137833
10.1161/01.RES.75.3.591
10.1152/physrev.00025.2003
10.1159/000074542
10.1016/j.bios.2006.10.003
10.2165/00002018-200427110-00002
10.1016/S0956-5663(01)00170-1
10.1016/S0956-5663(02)00155-0
10.1016/S1056-8719(01)00150-2
10.1109/TBME.1986.325894
10.1088/0967-3334/23/2/317
10.1126/science.1128640
10.1021/nl900096z
10.1002/smll.200800919
10.1038/nnano.2008.374
10.1007/s00216-005-3400-4
10.1557/mrs2007.47
10.1038/nbt1138
10.1038/nature05498
10.1073/pnas.0504146103
10.1073/pnas.0406159101
10.1007/s10439-005-9006-3
10.1126/science.1106587
10.1002/cphc.200301014
10.1002/adma.200500366
10.1021/nl070678d
10.1038/nprot.2006.227
10.1021/nl063056l
10.1016/j.bios.2007.08.027
10.1007/BF00370800
P Dayan, LF Abbott Theoretical Neuroscience :Computational and Mathematical Modeling of Neural Systems, eds TJ Sejnowski, T Poggio (MIT Press, Cambridge, MA), pp. 27–29 (2001). / Theoretical Neuroscience :Computational and Mathematical Modeling of Neural Systems by Dayan P (2001)

Dates

Type	When
Created	16 years, 4 months ago (April 13, 2009, 8:45 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 5:18 p.m.)
Indexed	4 weeks, 2 days ago (July 25, 2025, 6:09 a.m.)
Issued	16 years, 3 months ago (May 5, 2009)
Published	16 years, 3 months ago (May 5, 2009)
Published Online	16 years, 3 months ago (May 5, 2009)
Published Print	16 years, 3 months ago (May 5, 2009)

Funders 0

None

BibTeX

@article{Cohen_Karni_2009, title={Flexible electrical recording from cells using nanowire transistor arrays}, volume={106}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0902752106}, DOI={10.1073/pnas.0902752106}, number={18}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Cohen-Karni, Tzahi and Timko, Brian P. and Weiss, Lucien E. and Lieber, Charles M.}, year={2009}, month=may, pages={7309–7313} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        25
      ]
    ],
    "date-time": "2025-07-25T10:09:10Z",
    "timestamp": 1753438150961
  },
  "reference-count": 33,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "18",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2009,
        5,
        5
      ]
    ]
  },
  "abstract": "<jats:p>\n            Semiconductor nanowires (NWs) have unique electronic properties and sizes comparable with biological structures involved in cellular communication, thus making them promising nanostructures for establishing active interfaces with biological systems. We report a flexible approach to interface NW field-effect transistors (NWFETs) with cells and demonstrate this for silicon NWFET arrays coupled to embryonic chicken cardiomyocytes. Cardiomyocyte cells were cultured on thin, optically transparent polydimethylsiloxane (PDMS) sheets and then brought into contact with Si-NWFET arrays fabricated on standard substrates. NWFET conductance signals recorded from cardiomyocytes exhibited excellent signal-to-noise ratios with values routinely &gt;5 and signal amplitudes that were tuned by varying device sensitivity through changes in water gate\u2013voltage potential,\n            <jats:italic>\n              V\n              <jats:sub>g</jats:sub>\n            </jats:italic>\n            . Signals recorded from cardiomyocytes for\n            <jats:italic>\n              V\n              <jats:sub>g</jats:sub>\n            </jats:italic>\n            from \u22120.5 to +0.1 V exhibited amplitude variations from 31 to 7 nS whereas the calibrated voltage remained constant, indicating a robust NWFET/cell interface. In addition, signals recorded as a function of increasing/decreasing displacement of the PDMS/cell support to the device chip showed a reversible &gt;2\u00d7 increase in signal amplitude (calibrated voltage) from 31 nS (1.0 mV) to 72 nS (2.3 mV). Studies with the displacement close to but below the point of cell disruption yielded calibrated signal amplitudes as large as 10.5 \u00b1 0.2 mV. Last, multiplexed recording of signals from NWFET arrays interfaced to cardiomyocyte monolayers enabled temporal shifts and signal propagation to be determined with good spatial and temporal resolution. Our modular approach simplifies the process of interfacing cardiomyocytes and other cells to high-performance Si-NWFETs, thus increasing the experimental versatility of NWFET arrays and enabling device registration at the subcellular level.\n          </jats:p>",
  "DOI": "10.1073/pnas.0902752106",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2009,
        4,
        14
      ]
    ],
    "date-time": "2009-04-14T00:45:48Z",
    "timestamp": 1239669948000
  },
  "page": "7309-7313",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 201,
  "title": "Flexible electrical recording from cells using nanowire transistor arrays",
  "prefix": "10.1073",
  "volume": "106",
  "author": [
    {
      "given": "Tzahi",
      "family": "Cohen-Karni",
      "sequence": "first",
      "affiliation": [
        {
          "name": "School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138"
        }
      ]
    },
    {
      "given": "Brian P.",
      "family": "Timko",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Chemistry and Chemical Biology and"
        }
      ]
    },
    {
      "given": "Lucien E.",
      "family": "Weiss",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Chemistry and Chemical Biology and"
        }
      ]
    },
    {
      "given": "Charles M.",
      "family": "Lieber",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Chemistry and Chemical Biology and"
        },
        {
          "name": "School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        2009,
        5,
        5
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_3_1_2",
      "volume-title": "Ion Channels of Excitable Membranes",
      "author": "Hille B",
      "year": "2001",
      "unstructured": "B Hille Ion Channels of Excitable Membranes (Sinauer, Sunderland, MA, 2001)."
    },
    {
      "key": "e_1_3_3_2_2",
      "volume-title": "Cardiac Electrophysiology : From Cell to Bedside",
      "author": "Zipes DP",
      "year": "2004",
      "unstructured": "DP Zipes, J Jalife Cardiac Electrophysiology : From Cell to Bedside (Saunders, Philadelphia, 2004)."
    },
    {
      "key": "e_1_3_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/b137833"
    },
    {
      "key": "e_1_3_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1161/01.RES.75.3.591"
    },
    {
      "key": "e_1_3_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/physrev.00025.2003"
    },
    {
      "key": "e_1_3_3_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1159/000074542"
    },
    {
      "key": "e_1_3_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.bios.2006.10.003"
    },
    {
      "key": "e_1_3_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2165/00002018-200427110-00002"
    },
    {
      "key": "e_1_3_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0956-5663(01)00170-1"
    },
    {
      "key": "e_1_3_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0956-5663(02)00155-0"
    },
    {
      "key": "e_1_3_3_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1056-8719(01)00150-2"
    },
    {
      "key": "e_1_3_3_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1109/TBME.1986.325894"
    },
    {
      "key": "e_1_3_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1088/0967-3334/23/2/317"
    },
    {
      "key": "e_1_3_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1128640"
    },
    {
      "key": "e_1_3_3_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/nl900096z"
    },
    {
      "key": "e_1_3_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/smll.200800919"
    },
    {
      "key": "e_1_3_3_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nnano.2008.374"
    },
    {
      "key": "e_1_3_3_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s00216-005-3400-4"
    },
    {
      "key": "e_1_3_3_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1557/mrs2007.47"
    },
    {
      "key": "e_1_3_3_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nbt1138"
    },
    {
      "key": "e_1_3_3_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature05498"
    },
    {
      "key": "e_1_3_3_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0504146103"
    },
    {
      "key": "e_1_3_3_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0406159101"
    },
    {
      "key": "e_1_3_3_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s10439-005-9006-3"
    },
    {
      "key": "e_1_3_3_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1106587"
    },
    {
      "key": "e_1_3_3_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cphc.200301014"
    },
    {
      "key": "e_1_3_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/adma.200500366"
    },
    {
      "key": "e_1_3_3_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/nl070678d"
    },
    {
      "key": "e_1_3_3_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nprot.2006.227"
    },
    {
      "key": "e_1_3_3_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/nl063056l"
    },
    {
      "key": "e_1_3_3_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.bios.2007.08.027"
    },
    {
      "key": "e_1_3_3_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00370800"
    },
    {
      "key": "e_1_3_3_33_2",
      "first-page": "27",
      "volume-title": "Theoretical Neuroscience :Computational and Mathematical Modeling of Neural Systems",
      "author": "Dayan P",
      "year": "2001",
      "unstructured": "P Dayan, LF Abbott Theoretical Neuroscience :Computational and Mathematical Modeling of Neural Systems, eds TJ Sejnowski, T Poggio (MIT Press, Cambridge, MA), pp. 27\u201329 (2001)."
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.0902752106",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        12
      ]
    ],
    "date-time": "2022-04-12T21:18:30Z",
    "timestamp": 1649798310000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.0902752106"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2009,
        5,
        5
      ]
    ]
  },
  "references-count": 33,
  "journal-issue": {
    "issue": "18",
    "published-print": {
      "date-parts": [
        [
          2009,
          5,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.0902752106"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.0902752106",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        2009,
        5,
        5
      ]
    ]
  },
  "assertion": [
    {
      "value": "2009-03-05",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2009-05-05",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}