DOI: 10.1073/pnas.0901631106. Structure of a mitochondrial ribosome with minimal RNA

Structure of a mitochondrial ribosome with minimal RNA

10.1073/pnas.0901631106

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The Leishmania tarentolae mitochondrial ribosome (Lmr) is a minimal ribosomal RNA (rRNA)-containing ribosome. We have obtained a cryo-EM map of the Lmr. The map reveals several features that have not been seen in previously-determined structures of eubacterial or eukaryotic (cytoplasmic or organellar) ribosomes to our knowledge. Comparisons of the Lmr map with X-ray crystallographic and cryo-EM maps of the eubacterial ribosomes and a cryo-EM map of the mammalian mitochondrial ribosome show that ( i ) the overall structure of the Lmr is considerably more porous, ( ii ) the topology of the intersubunit space is significantly different, with fewer intersubunit bridges, but more tunnels, and ( iii ) several of the functionally-important rRNA regions, including the α-sarcin-ricin loop, have different relative positions within the structure. Furthermore, the major portions of the mRNA channel, the tRNA passage, and the nascent polypeptide exit tunnel contain Lmr-specific proteins, suggesting that the mechanisms for mRNA recruitment, tRNA interaction, and exiting of the nascent polypeptide in Lmr must differ markedly from the mechanisms deduced for ribosomes in other organisms. Our study identifies certain structural features that are characteristic solely of mitochondrial ribosomes and other features that are characteristic of both mitochondrial and chloroplast ribosomes (i.e., organellar ribosomes).

Bibliography

Sharma, M. R., Booth, T. M., Simpson, L., Maslov, D. A., & Agrawal, R. K. (2009). Structure of a mitochondrial ribosome with minimal RNA. Proceedings of the National Academy of Sciences, 106(24), 9637â9642.

Authors 5

Manjuli R. Sharma (first)
Timothy M. Booth (additional)
Larry Simpson (additional)
Dmitri A. Maslov (additional)
Rajendra K. Agrawal (additional)

References 40 Referenced 78

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Dates

Type	When
Created	16 years, 2 months ago (June 4, 2009, 6:34 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 6 p.m.)
Indexed	1 month ago (July 26, 2025, 5:21 a.m.)
Issued	16 years, 2 months ago (June 16, 2009)
Published	16 years, 2 months ago (June 16, 2009)
Published Online	16 years, 2 months ago (June 16, 2009)
Published Print	16 years, 2 months ago (June 16, 2009)

Funders 0

None

BibTeX

@article{Sharma_2009, title={Structure of a mitochondrial ribosome with minimal RNA}, volume={106}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0901631106}, DOI={10.1073/pnas.0901631106}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sharma, Manjuli R. and Booth, Timothy M. and Simpson, Larry and Maslov, Dmitri A. and Agrawal, Rajendra K.}, year={2009}, month=jun, pages={9637–9642} }

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