DOI: 10.1073/pnas.090104997. Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies of the denaturation of chymotrypsin inhibitor 2

Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies of the denaturation of chymotrypsin inhibitor 2

10.1073/pnas.090104997

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We report single-molecule folding studies of a small, single-domain protein, chymotrypsin inhibitor 2 (CI2). CI2 is an excellent model system for protein folding studies and has been extensively studied, both experimentally (at the ensemble level) and theoretically. Conformationally assisted ligation methodology was used to synthesize the proteins and site-specifically label them with donor and acceptor dyes. Folded and denatured subpopulations were observed by fluorescence resonance energy transfer (FRET) measurements on freely diffusing single protein molecules. Properties of these subpopulations were directly monitored as a function of guanidinium chloride concentration. It is shown that new information about different aspects of the protein folding reaction can be extracted from such subpopulation properties. Shifts in the mean transfer efficiencies are discussed, FRET efficiency distributions are translated into potentials, and denaturation curves are directly plotted from the areas of the FRET peaks. Changes in stability caused by mutation also are measured by comparing pseudo wild-type CI2 with a destabilized mutant (K17G). Current limitations and future possibilities and prospects for single-pair FRET protein folding investigations are discussed.

Bibliography

Deniz, A. A., Laurence, T. A., Beligere, G. S., Dahan, M., Martin, A. B., Chemla, D. S., Dawson, P. E., Schultz, P. G., & Weiss, S. (2000). Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies of the denaturation of chymotrypsin inhibitor 2. Proceedings of the National Academy of Sciences, 97(10), 5179â5184.

Authors 9

Ashok A. Deniz (first)
Ted A. Laurence (additional)
Gangamani S. Beligere (additional)
Maxime Dahan (additional)
Andrew B. Martin (additional)
Daniel S. Chemla (additional)
Philip E. Dawson (additional)
Peter G. Schultz (additional)
Shimon Weiss (additional)

References 36 Referenced 408

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:38 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 5:37 p.m.)
Indexed	4 weeks, 1 day ago (Aug. 6, 2025, 9:52 a.m.)
Issued	25 years, 4 months ago (May 2, 2000)
Published	25 years, 4 months ago (May 2, 2000)
Published Online	25 years, 4 months ago (May 2, 2000)
Published Print	25 years, 3 months ago (May 9, 2000)

Funders 0

None

BibTeX

@article{Deniz_2000, title={Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies of the denaturation of chymotrypsin inhibitor 2}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.090104997}, DOI={10.1073/pnas.090104997}, number={10}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Deniz, Ashok A. and Laurence, Ted A. and Beligere, Gangamani S. and Dahan, Maxime and Martin, Andrew B. and Chemla, Daniel S. and Dawson, Philip E. and Schultz, Peter G. and Weiss, Shimon}, year={2000}, month=may, pages={5179–5184} }

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