DOI: 10.1073/pnas.090083797. The long-range supraorganization of the bacterial photosynthetic unit: A key role for PufX

The long-range supraorganization of the bacterial photosynthetic unit: A key role for PufX

10.1073/pnas.090083797

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Bacterial photosynthesis relies on the interplay between light harvesting and electron transfer complexes, all of which are located within the intracytoplasmic membrane. These complexes capture and transfer solar energy, which is used to generate a proton gradient. In this study, we identify one of the factors that determines the organization of these complexes. We undertook a comparison of the organization of the light-harvesting complex 1 (LH1)/reaction center (RC) cores in the LH2 − mutant of Rhodobacter sphaeroides in the presence or absence of the PufX protein. From polarized absorption spectra on oriented membranes, we conclude that PufX induces a specific orientation of the reaction center in the LH1 ring, as well as the formation of a long-range regular array of LH1-RC cores in the photosynthetic membrane. From our data, we have constructed a precise model of how the RC is positioned within the LH1 ring relative to the long (orientation) axis of the photosynthetic membrane.

Bibliography

Frese, R. N., Olsen, J. D., Branvall, R., Westerhuis, W. H. J., Hunter, C. N., & van Grondelle, R. (2000). The long-range supraorganization of the bacterial photosynthetic unit: A key role for PufX. Proceedings of the National Academy of Sciences, 97(10), 5197â5202.

Authors 6

Raoul N. Frese (first)
John D. Olsen (additional)
Rikard Branvall (additional)
Willem H. J. Westerhuis (additional)
C. Neil Hunter (additional)
Rienk van Grondelle (additional)

References 25 Referenced 79

10.1038/318618a0
10.1038/374517a0
10.1126/science.277.5322.60
10.1002/j.1460-2075.1995.tb07041.x
10.1006/jmbi.1998.2050
10.1128/jb.172.2.977-985.1990
10.1016/0005-2728(92)90077-F
10.1021/bi00046a033
10.1016/0014-5793(94)00701-2
10.1074/jbc.271.6.3285
10.1016/S0021-9258(19)47309-2
10.1007/BF00040996
10.1021/bi982891h
R J Pugh, P McGlynn, M R Jones, C N Hunter Biochim Biophys Acta 1366, 302–316 (1998). / Biochim Biophys Acta by Pugh R J (1998)
10.1006/jmbi.1996.0714
10.1093/emboj/18.3.534
10.1111/j.1365-2958.1992.tb01556.x
10.1021/bi00133a011
10.1073/pnas.91.15.7124
10.1021/jp991816t
10.1016/0005-2728(85)90138-0
10.1016/0005-2728(84)90009-4
10.1021/bi00409a050
10.1128/jb.170.3.1103-1115.1988
10.1111/j.1365-2958.1992.tb01773.x

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:38 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 5:36 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 6, 2025, 9:48 a.m.)
Issued	25 years, 3 months ago (May 2, 2000)
Published	25 years, 3 months ago (May 2, 2000)
Published Online	25 years, 3 months ago (May 2, 2000)
Published Print	25 years, 3 months ago (May 9, 2000)

Funders 0

None

BibTeX

@article{Frese_2000, title={The long-range supraorganization of the bacterial photosynthetic unit: A key role for PufX}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.090083797}, DOI={10.1073/pnas.090083797}, number={10}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Frese, Raoul N. and Olsen, John D. and Branvall, Rikard and Westerhuis, Willem H. J. and Hunter, C. Neil and van Grondelle, Rienk}, year={2000}, month=may, pages={5197–5202} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        6
      ]
    ],
    "date-time": "2025-08-06T13:48:19Z",
    "timestamp": 1754488099820
  },
  "reference-count": 25,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "10",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2000,
        5,
        9
      ]
    ]
  },
  "abstract": "<jats:p>\n            Bacterial photosynthesis relies on the interplay between light harvesting and electron transfer complexes, all of which are located within the intracytoplasmic membrane. These complexes capture and transfer solar energy, which is used to generate a proton gradient. In this study, we identify one of the factors that determines the organization of these complexes. We undertook a comparison of the organization of the light-harvesting complex 1 (LH1)/reaction center (RC) cores in the LH2\n            <jats:sup>\u2212</jats:sup>\n            mutant of\n            <jats:italic>Rhodobacter sphaeroides</jats:italic>\n            in the presence or absence of the PufX protein. From polarized absorption spectra on oriented membranes, we conclude that PufX induces a specific orientation of the reaction center in the LH1 ring, as well as the formation of a long-range regular array of LH1-RC cores in the photosynthetic membrane. From our data, we have constructed a precise model of how the RC is positioned within the LH1 ring relative to the long (orientation) axis of the photosynthetic membrane.\n          </jats:p>",
  "DOI": "10.1073/pnas.090083797",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T14:38:21Z",
    "timestamp": 1027694301000
  },
  "page": "5197-5202",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 79,
  "title": "The long-range supraorganization of the bacterial photosynthetic unit: A key role for PufX",
  "prefix": "10.1073",
  "volume": "97",
  "author": [
    {
      "given": "Raoul N.",
      "family": "Frese",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Physics and Astronomy and Institute for Molecular Biological Sciences, Vrije Universiteit, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands; and Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2UH, United Kingdom"
        }
      ]
    },
    {
      "given": "John D.",
      "family": "Olsen",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Physics and Astronomy and Institute for Molecular Biological Sciences, Vrije Universiteit, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands; and Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2UH, United Kingdom"
        }
      ]
    },
    {
      "given": "Rikard",
      "family": "Branvall",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Physics and Astronomy and Institute for Molecular Biological Sciences, Vrije Universiteit, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands; and Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2UH, United Kingdom"
        }
      ]
    },
    {
      "given": "Willem H. J.",
      "family": "Westerhuis",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Physics and Astronomy and Institute for Molecular Biological Sciences, Vrije Universiteit, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands; and Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2UH, United Kingdom"
        }
      ]
    },
    {
      "given": "C. Neil",
      "family": "Hunter",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Physics and Astronomy and Institute for Molecular Biological Sciences, Vrije Universiteit, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands; and Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2UH, United Kingdom"
        }
      ]
    },
    {
      "given": "Rienk",
      "family": "van Grondelle",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Physics and Astronomy and Institute for Molecular Biological Sciences, Vrije Universiteit, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands; and Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2UH, United Kingdom"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        2000,
        5,
        2
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_4_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/318618a0"
    },
    {
      "key": "e_1_3_4_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/374517a0"
    },
    {
      "key": "e_1_3_4_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.277.5322.60"
    },
    {
      "key": "e_1_3_4_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1995.tb07041.x"
    },
    {
      "key": "e_1_3_4_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1998.2050"
    },
    {
      "key": "e_1_3_4_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.172.2.977-985.1990"
    },
    {
      "key": "e_1_3_4_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(92)90077-F"
    },
    {
      "key": "e_1_3_4_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00046a033"
    },
    {
      "key": "e_1_3_4_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(94)00701-2"
    },
    {
      "key": "e_1_3_4_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.6.3285"
    },
    {
      "key": "e_1_3_4_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)47309-2"
    },
    {
      "key": "e_1_3_4_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00040996"
    },
    {
      "key": "e_1_3_4_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi982891h"
    },
    {
      "key": "e_1_3_4_14_2",
      "first-page": "302",
      "volume": "1366",
      "author": "Pugh R J",
      "year": "1998",
      "unstructured": "R J Pugh, P McGlynn, M R Jones, C N Hunter Biochim Biophys Acta 1366, 302\u2013316 (1998).",
      "journal-title": "Biochim Biophys Acta"
    },
    {
      "key": "e_1_3_4_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1996.0714"
    },
    {
      "key": "e_1_3_4_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/18.3.534"
    },
    {
      "key": "e_1_3_4_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.1992.tb01556.x"
    },
    {
      "key": "e_1_3_4_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00133a011"
    },
    {
      "key": "e_1_3_4_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.91.15.7124"
    },
    {
      "key": "e_1_3_4_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/jp991816t"
    },
    {
      "key": "e_1_3_4_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(85)90138-0"
    },
    {
      "key": "e_1_3_4_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(84)90009-4"
    },
    {
      "key": "e_1_3_4_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00409a050"
    },
    {
      "key": "e_1_3_4_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.170.3.1103-1115.1988"
    },
    {
      "key": "e_1_3_4_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.1992.tb01773.x"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.090083797",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        12
      ]
    ],
    "date-time": "2022-04-12T21:36:48Z",
    "timestamp": 1649799408000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.090083797"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2000,
        5,
        2
      ]
    ]
  },
  "references-count": 25,
  "journal-issue": {
    "issue": "10",
    "published-print": {
      "date-parts": [
        [
          2000,
          5,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.090083797"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.090083797",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        2000,
        5,
        2
      ]
    ]
  },
  "assertion": [
    {
      "value": "1999-10-13",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2000-02-28",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2000-05-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}