DOI: 10.1073/pnas.0832180100. Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles

Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles

10.1073/pnas.0832180100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

HIV pseudotypes bearing native hepatitis C virus (HCV) glycoproteins (strain H and Con1) are infectious for the human hepatoma cell lines Huh-7 and PLC/PR5. Infectivity depends on coexpression of both E1 and E2 glycoproteins, is pH-dependent, and can be neutralized by mAbs mapping to amino acids 412–447 within E2. Cell-surface expression of one or all of the candidate receptor molecules (CD81, low-density lipoprotein receptor, scavenger receptor class B type 1, and dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin) failed to confer permissivity to HIV–HCV pseudotype infection. However, HIV–HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex.

Bibliography

Hsu, M., Zhang, J., Flint, M., Logvinoff, C., Cheng-Mayer, C., Rice, C. M., & McKeating, J. A. (2003). Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles. Proceedings of the National Academy of Sciences, 100(12), 7271â7276.

Authors 7

Mayla Hsu (first)
Jie Zhang (additional)
Mike Flint (additional)
Carine Logvinoff (additional)
Cecilia Cheng-Mayer (additional)
Charles M. Rice (additional)
Jane A. McKeating (additional)

References 29 Referenced 666

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Dates

Type	When
Created	22 years, 2 months ago (June 10, 2003, 1:06 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 6:08 p.m.)
Indexed	1 month, 1 week ago (July 19, 2025, 11:27 p.m.)
Issued	22 years, 3 months ago (May 21, 2003)
Published	22 years, 3 months ago (May 21, 2003)
Published Online	22 years, 3 months ago (May 21, 2003)
Published Print	22 years, 2 months ago (June 10, 2003)

Funders 0

None

BibTeX

@article{Hsu_2003, title={Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0832180100}, DOI={10.1073/pnas.0832180100}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hsu, Mayla and Zhang, Jie and Flint, Mike and Logvinoff, Carine and Cheng-Mayer, Cecilia and Rice, Charles M. and McKeating, Jane A.}, year={2003}, month=may, pages={7271–7276} }

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