DOI: 10.1073/pnas.0831078100. Dichloroacetate effects on glucose and lactate oxidation by neurons and astroglia <i>in vitro</i> and on glucose utilization by brain <i>in vivo</i>

Dichloroacetate effects on glucose and lactate oxidation by neurons and astroglia in vitro and on glucose utilization by brain in vivo

10.1073/pnas.0831078100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Neuronal cultures in vitro readily oxidized both D-[ 14 C]glucose and l -[ 14 C]lactate to 14 CO 2 , whereas astroglial cultures oxidized both substrates sparingly and metabolized glucose predominantly to lactate and released it into the medium. [ 14 C]Glucose oxidation to 14 CO 2 varied inversely with unlabeled lactate concentration in the medium, particularly in neurons, and increased progressively with decreasing lactate concentration. Adding unlabeled glucose to the medium inhibited [ 14 C]lactate oxidation to 14 CO 2 only in astroglia but not in neurons, indicating a kinetic preference in neurons for oxidation of extracellular lactate over intracellular pyruvate/lactate produced by glycolysis. Protein kinase-catalyzed phosphorylation inactivates pyruvate dehydrogenase (PDH), which regulates pyruvate entry into the tricarboxylic acid cycle. Dichloroacetate inhibits this kinase, thus enhancing PDH activity. In vitro dichloroacetate stimulated glucose and lactate oxidation to CO 2 and reduced lactate release mainly in astroglia, indicating that limitations in glucose and lactate oxidation by astroglia may be due to a greater balance of PDH toward the inactive form. To assess the significance of astroglial export of lactate to neurons in vivo , we attempted to diminish this traffic in rats by administering dichloroacetate (50 mg/kg) intravenously to stimulate astroglial lactate oxidation and then examined the effects on baseline and functionally activated local cerebral glucose utilization (lCMR glc ). Dichloroacetate raised baseline lCMR glc throughout the brain and decreased the percent increases in lCMR glc evoked by functional activation. These studies provide evidence in support of the compartmentalization of glucose metabolism between astroglia and neurons but indicate that the compartmentalization may be neither complete nor entirely obligatory.

Bibliography

Itoh, Y., Esaki, T., Shimoji, K., Cook, M., Law, M. J., Kaufman, E., & Sokoloff, L. (2003). Dichloroacetate effects on glucose and lactate oxidation by neurons and astroglia in vitro and on glucose utilization by brain in vivo. Proceedings of the National Academy of Sciences, 100(8), 4879â4884.

Authors 7

Yoshiaki Itoh (first)
Takanori Esaki (additional)
Kazuaki Shimoji (additional)
Michelle Cook (additional)
Mona J. Law (additional)
Elaine Kaufman (additional)
Louis Sokoloff (additional)

References 28 Referenced 246

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Dates

Type	When
Created	22 years, 4 months ago (April 15, 2003, 6:56 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 5:42 p.m.)
Indexed	1 month, 1 week ago (July 26, 2025, 5:16 a.m.)
Issued	22 years, 5 months ago (March 31, 2003)
Published	22 years, 5 months ago (March 31, 2003)
Published Online	22 years, 5 months ago (March 31, 2003)
Published Print	22 years, 4 months ago (April 15, 2003)

Funders 0

None

BibTeX

@article{Itoh_2003, title={Dichloroacetate effects on glucose and lactate oxidation by neurons and astroglia in vitro and on glucose utilization by brain in vivo}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0831078100}, DOI={10.1073/pnas.0831078100}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Itoh, Yoshiaki and Esaki, Takanori and Shimoji, Kazuaki and Cook, Michelle and Law, Mona J. and Kaufman, Elaine and Sokoloff, Louis}, year={2003}, month=mar, pages={4879–4884} }

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