DOI: 10.1073/pnas.0830856100. An empty <i>Drosophila</i> stem cell niche reactivates the proliferation of ectopic cells

An empty Drosophila stem cell niche reactivates the proliferation of ectopic cells

10.1073/pnas.0830856100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Stem cells are thought to reside in regulatory microenvironments (“niches”) generated by stable stromal neighbors. To investigate the significance of empty niches vacated by stem cell loss, we studied Drosophila ovarioles, which maintain two to three germ-line stem cells in a niche requiring adhesive stromal cap cells and Decapentaplegic signals. After experimentally emptying the germ-line stem cell niche, cap cell activity persists for several weeks. Initially, somatic inner germarium sheath cells enter the empty niche, respond to Dpp, but fail to divide. Subsequently, follicle cell progenitors, including somatic stem cells enter the niche, respond to Dpp, and proliferate as long as cap cells remain. Proliferation requires the normal hedgehog signal of the somatic stem cells as well as proximity to the niche. Thus, empty niches can persist, signal incoming cells, and support ectopic proliferation. Similar events may underlie some disease states.

Bibliography

Kai, T., & Spradling, A. (2003). An empty Drosophila stem cell niche reactivates the proliferation of ectopic cells. Proceedings of the National Academy of Sciences, 100(8), 4633â4638.

Authors 2

Toshie Kai (first)
Allan Spradling (additional)

References 28 Referenced 246

10.1126/science.287.5457.1427
10.1038/35102160
10.1126/science.290.5490.328
10.1073/pnas.91.24.11298
10.1038/416854a
10.1016/S0092-8674(01)00208-2
10.1038/43919
10.1126/science.290.5497.1775
10.1038/81326
10.1038/sj.onc.1205604
R S King Ovarian Development in Drosophila melanogaster (Academic, New York, 1970). / Ovarian Development in Drosophila melanogaster by King R S (1970)
T Xie, A C Spradling Stem Cells, eds D Marshak, R Gardner, D Gottlieb (Cold Spring Harbor Lab. Press, Plainview, NY), pp. 129–148 (2001). / Stem Cells by Xie T (2001)
10.1126/science.1069871
10.1016/S0092-8674(00)81424-5
10.1016/S1097-2765(01)00197-6
10.1242/dev.121.11.3797
10.1242/dev.122.4.1125
10.1038/35069099
A C Spradling The Development of Drosophila melanogaster, eds M Bate, A Martizen-Arias (Cold Spring Lab. Harbor Press, Plainview, NY), pp. 1–70 (1993). / The Development of Drosophila melanogaster by Spradling A C (1993)
10.1093/genetics/132.3.737
10.1002/(SICI)1097-0177(199707)209:3<310::AID-AJA6>3.0.CO;2-L
10.1016/S1097-2765(00)80403-7
10.1038/39362
10.1242/dev.124.18.3651
C J Fielding J Embryol Exp Morphol 17, 375–384 (1967). / J Embryol Exp Morphol by Fielding C J (1967)
10.1007/BF00868697
10.1016/0012-1606(82)90019-7
10.1073/pnas.232389399

Dates

Type	When
Created	22 years, 4 months ago (April 15, 2003, 6:56 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 5:41 p.m.)
Indexed	1 day, 22 hours ago (Aug. 27, 2025, 11:46 a.m.)
Issued	22 years, 4 months ago (April 3, 2003)
Published	22 years, 4 months ago (April 3, 2003)
Published Online	22 years, 4 months ago (April 3, 2003)
Published Print	22 years, 4 months ago (April 15, 2003)

Funders 0

None

BibTeX

@article{Kai_2003, title={An empty Drosophila stem cell niche reactivates the proliferation of ectopic cells}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0830856100}, DOI={10.1073/pnas.0830856100}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kai, Toshie and Spradling, Allan}, year={2003}, month=apr, pages={4633–4638} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        27
      ]
    ],
    "date-time": "2025-08-27T15:46:23Z",
    "timestamp": 1756309583359
  },
  "reference-count": 28,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "8",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2003,
        4,
        15
      ]
    ]
  },
  "abstract": "<jats:p>\n            Stem cells are thought to reside in regulatory microenvironments (\u201cniches\u201d) generated by stable stromal neighbors. To investigate the significance of empty niches vacated by stem cell loss, we studied\n            <jats:italic>Drosophila</jats:italic>\n            ovarioles, which maintain two to three germ-line stem cells in a niche requiring adhesive stromal cap cells and\n            <jats:italic>Decapentaplegic</jats:italic>\n            signals. After experimentally emptying the germ-line stem cell niche, cap cell activity persists for several weeks. Initially, somatic inner germarium sheath cells enter the empty niche, respond to Dpp, but fail to divide. Subsequently, follicle cell progenitors, including somatic stem cells enter the niche, respond to Dpp, and proliferate as long as cap cells remain. Proliferation requires the normal\n            <jats:italic>hedgehog</jats:italic>\n            signal of the somatic stem cells as well as proximity to the niche. Thus, empty niches can persist, signal incoming cells, and support ectopic proliferation. Similar events may underlie some disease states.\n          </jats:p>",
  "DOI": "10.1073/pnas.0830856100",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        4,
        15
      ]
    ],
    "date-time": "2003-04-15T22:56:03Z",
    "timestamp": 1050447363000
  },
  "page": "4633-4638",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 246,
  "title": "An empty\n            <i>Drosophila</i>\n            stem cell niche reactivates the proliferation of ectopic cells",
  "prefix": "10.1073",
  "volume": "100",
  "author": [
    {
      "given": "Toshie",
      "family": "Kai",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Embryology, The Howard Hughes Medical Institute Research Laboratories, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210"
        }
      ]
    },
    {
      "given": "Allan",
      "family": "Spradling",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Embryology, The Howard Hughes Medical Institute Research Laboratories, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        2003,
        4,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_3_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.287.5457.1427"
    },
    {
      "key": "e_1_3_3_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35102160"
    },
    {
      "key": "e_1_3_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.290.5490.328"
    },
    {
      "key": "e_1_3_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.91.24.11298"
    },
    {
      "key": "e_1_3_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/416854a"
    },
    {
      "key": "e_1_3_3_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(01)00208-2"
    },
    {
      "key": "e_1_3_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/43919"
    },
    {
      "key": "e_1_3_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.290.5497.1775"
    },
    {
      "key": "e_1_3_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/81326"
    },
    {
      "key": "e_1_3_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.onc.1205604"
    },
    {
      "key": "e_1_3_3_11_2",
      "volume-title": "Ovarian Development in Drosophila melanogaster",
      "author": "King R S",
      "year": "1970",
      "unstructured": "R S King Ovarian Development in Drosophila melanogaster (Academic, New York, 1970)."
    },
    {
      "key": "e_1_3_3_12_2",
      "first-page": "129",
      "volume-title": "Stem Cells",
      "author": "Xie T",
      "year": "2001",
      "unstructured": "T Xie, A C Spradling Stem Cells, eds D Marshak, R Gardner, D Gottlieb (Cold Spring Harbor Lab. Press, Plainview, NY), pp. 129\u2013148 (2001)."
    },
    {
      "key": "e_1_3_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1069871"
    },
    {
      "key": "e_1_3_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81424-5"
    },
    {
      "key": "e_1_3_3_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(01)00197-6"
    },
    {
      "key": "e_1_3_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.121.11.3797"
    },
    {
      "key": "e_1_3_3_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.122.4.1125"
    },
    {
      "key": "e_1_3_3_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35069099"
    },
    {
      "key": "e_1_3_3_19_2",
      "first-page": "1",
      "volume-title": "The Development of Drosophila melanogaster",
      "author": "Spradling A C",
      "year": "1993",
      "unstructured": "A C Spradling The Development of Drosophila melanogaster, eds M Bate, A Martizen-Arias (Cold Spring Lab. Harbor Press, Plainview, NY), pp. 1\u201370 (1993)."
    },
    {
      "key": "e_1_3_3_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/genetics/132.3.737"
    },
    {
      "key": "e_1_3_3_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1097-0177(199707)209:3<310::AID-AJA6>3.0.CO;2-L"
    },
    {
      "key": "e_1_3_3_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(00)80403-7"
    },
    {
      "key": "e_1_3_3_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/39362"
    },
    {
      "key": "e_1_3_3_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/dev.124.18.3651"
    },
    {
      "key": "e_1_3_3_25_2",
      "first-page": "375",
      "volume": "17",
      "author": "Fielding C J",
      "year": "1967",
      "unstructured": "C J Fielding J Embryol Exp Morphol 17, 375\u2013384 (1967).",
      "journal-title": "J Embryol Exp Morphol"
    },
    {
      "key": "e_1_3_3_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00868697"
    },
    {
      "key": "e_1_3_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0012-1606(82)90019-7"
    },
    {
      "key": "e_1_3_3_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.232389399"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.0830856100",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        12
      ]
    ],
    "date-time": "2022-04-12T21:41:51Z",
    "timestamp": 1649799711000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.0830856100"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2003,
        4,
        3
      ]
    ]
  },
  "references-count": 28,
  "journal-issue": {
    "issue": "8",
    "published-print": {
      "date-parts": [
        [
          2003,
          4,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.0830856100"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.0830856100",
  "relation": {
    "has-review": [
      {
        "id-type": "doi",
        "id": "10.3410/f.1013015.190523",
        "asserted-by": "object"
      },
      {
        "id-type": "doi",
        "id": "10.3410/f.1013015.188336",
        "asserted-by": "object"
      }
    ]
  },
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        2003,
        4,
        3
      ]
    ]
  },
  "assertion": [
    {
      "value": "2003-02-12",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2003-04-03",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}