DOI: 10.1073/pnas.082644099. Inhibition of chymotrypsin through surface binding using nanoparticle-based receptors

Inhibition of chymotrypsin through surface binding using nanoparticle-based receptors

10.1073/pnas.082644099

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Efficient binding of biomacromolecular surfaces by synthetic systems requires the effective presentation of complementary elements over large surface areas. We demonstrate here the use of mixed monolayer protected gold clusters (MMPCs) as scaffolds for the binding and inhibition of chymotrypsin. In these studies anionically functionalized amphiphilic MMPCs were shown to inhibit chymotrypsin through a two-stage mechanism featuring fast reversible inhibition followed by a slower irreversible process. This interaction is very efficient, with aK\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{_{i}^{app}}}\end{equation*}\end{document}= 10.4 ± 1.3 nM. The MMPC–protein complex was characterized by CD, demonstrating an almost complete denaturation of the enzyme over time. Dynamic light scattering studies confirm that inhibition proceeds without substantial MMPC aggregation. The electrostatic nature of the engineered interactions provides a level of selectivity: little or no inhibition of function was observed with elastase, β-galactosidase, or cellular retinoic acid binding protein.

Bibliography

Fischer, N. O., McIntosh, C. M., Simard, J. M., & Rotello, V. M. (2002). Inhibition of chymotrypsin through surface binding using nanoparticle-based receptors. Proceedings of the National Academy of Sciences, 99(8), 5018â5023.

Authors 4

Nicholas O. Fischer (first)
Catherine M. McIntosh (additional)
Joseph M. Simard (additional)
Vincent M. Rotello (additional)

References 40 Referenced 167

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:45 a.m.)
Deposited	1 year, 7 months ago (Jan. 3, 2024, 11:57 p.m.)
Indexed	2 days, 13 hours ago (Aug. 27, 2025, 11:46 a.m.)
Issued	23 years, 4 months ago (April 2, 2002)
Published	23 years, 4 months ago (April 2, 2002)
Published Online	23 years, 4 months ago (April 2, 2002)
Published Print	23 years, 4 months ago (April 16, 2002)

Funders 0

None

BibTeX

@article{Fischer_2002, title={Inhibition of chymotrypsin through surface binding using nanoparticle-based receptors}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.082644099}, DOI={10.1073/pnas.082644099}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Fischer, Nicholas O. and McIntosh, Catherine M. and Simard, Joseph M. and Rotello, Vincent M.}, year={2002}, month=apr, pages={5018–5023} }

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