DOI: 10.1073/pnas.082117599. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells

A DNA vector-based RNAi technology to suppress gene expression in mammalian cells

10.1073/pnas.082117599

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful reverse genetic tool to silence gene expression in multiple organisms including plants, Caenorhabditis elegans , and Drosophila . The discovery that synthetic double-stranded, 21-nt small interfering RNA triggers gene-specific silencing in mammalian cells has further expanded the utility of RNAi into mammalian systems. Here we report a technology that allows synthesis of small interfering RNAs from DNA templates in vivo to efficiently inhibit endogenous gene expression. Significantly, we were able to use this approach to demonstrate, in multiple cell lines, robust inhibition of several endogenous genes of diverse functions. These findings highlight the general utility of this DNA vector-based RNAi technology in suppressing gene expression in mammalian cells.

Bibliography

Sui, G., Soohoo, C., Affar, E. B., Gay, F., Shi, Y., Forrester, W. C., & Shi, Y. (2002). A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proceedings of the National Academy of Sciences, 99(8), 5515â5520.

Authors 7

Guangchao Sui (first)
Christina Soohoo (additional)
El Bachir Affar (additional)
Frédérique Gay (additional)
Yujiang Shi (additional)
William C. Forrester (additional)
Yang Shi (additional)

References 22 Referenced 888

10.1038/35888
E Bernstein, A M Denli, G J Hannon RNA 7, 1509–1521 (2001). / RNA by Bernstein E (2001)
10.1101/gad.880001
10.1038/nsb0901-746
10.1073/pnas.060034297
10.1073/pnas.96.4.1451
10.1101/gad.13.24.3191
C Baglioni, T W Nilsen Interferon 5, 23–42 (1983). / Interferon by Baglioni C (1983)
10.1042/bst0250509
10.1073/pnas.032652399
10.1073/pnas.261562698
10.1038/35078107
10.1016/0092-8674(80)90385-2
10.1101/gad.862301
10.1038/72850
10.1038/78531
10.1038/35053110
10.1101/gad.927801
10.1126/science.1062039
10.1073/pnas.83.22.8575
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10.1016/0092-8674(92)90611-F

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:45 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 6:25 p.m.)
Indexed	3 days, 14 hours ago (Aug. 19, 2025, 6:27 a.m.)
Issued	23 years, 4 months ago (April 16, 2002)
Published	23 years, 4 months ago (April 16, 2002)
Published Online	23 years, 4 months ago (April 16, 2002)
Published Print	23 years, 4 months ago (April 16, 2002)

Funders 0

None

BibTeX

@article{Sui_2002, title={A DNA vector-based RNAi technology to suppress gene expression in mammalian cells}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.082117599}, DOI={10.1073/pnas.082117599}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sui, Guangchao and Soohoo, Christina and Affar, El Bachir and Gay, Frédérique and Shi, Yujiang and Forrester, William C. and Shi, Yang}, year={2002}, month=apr, pages={5515–5520} }

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