DOI: 10.1073/pnas.0813360106. Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin

Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin

10.1073/pnas.0813360106

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Protein secretion is a common property of pathogenic microbes. Gram-negative bacterial pathogens use at least 6 distinct extracellular protein secretion systems to export proteins through their multilayered cell envelope and in some cases into host cells. Among the most widespread is the newly recognized Type VI secretion system (T6SS) which is composed of 15–20 proteins whose biochemical functions are not well understood. Using crystallographic, biochemical, and bioinformatic analyses, we identified 3 T6SS components, which are homologous to bacteriophage tail proteins. These include the tail tube protein; the membrane-penetrating needle, situated at the distal end of the tube; and another protein associated with the needle and tube. We propose that T6SS is a multicomponent structure whose extracellular part resembles both structurally and functionally a bacteriophage tail, an efficient machine that translocates proteins and DNA across lipid membranes into cells.

Bibliography

Leiman, P. G., Basler, M., Ramagopal, U. A., Bonanno, J. B., Sauder, J. M., Pukatzki, S., Burley, S. K., Almo, S. C., & Mekalanos, J. J. (2009). Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin. Proceedings of the National Academy of Sciences, 106(11), 4154â4159.

Authors 9

Petr G. Leiman (first)
Marek Basler (additional)
Udupi A. Ramagopal (additional)
Jeffrey B. Bonanno (additional)
J. Michael Sauder (additional)
Stefan Pukatzki (additional)
Stephen K. Burley (additional)
Steven C. Almo (additional)
John J. Mekalanos (additional)

References 44 Referenced 556

Dates

Type	When
Created	16 years, 5 months ago (Feb. 27, 2009, 9:11 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 5:28 p.m.)
Indexed	1 day, 9 hours ago (Aug. 24, 2025, 6:57 p.m.)
Issued	16 years, 5 months ago (March 17, 2009)
Published	16 years, 5 months ago (March 17, 2009)
Published Online	16 years, 5 months ago (March 17, 2009)
Published Print	16 years, 5 months ago (March 17, 2009)

Funders 0

None

BibTeX

@article{Leiman_2009, title={Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin}, volume={106}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0813360106}, DOI={10.1073/pnas.0813360106}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Leiman, Petr G. and Basler, Marek and Ramagopal, Udupi A. and Bonanno, Jeffrey B. and Sauder, J. Michael and Pukatzki, Stefan and Burley, Stephen K. and Almo, Steven C. and Mekalanos, John J.}, year={2009}, month=mar, pages={4154–4159} }

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