DOI: 10.1073/pnas.0812071106. Atomic structure reveals the unique capsid organization of a dsRNA virus

Atomic structure reveals the unique capsid organization of a dsRNA virus

10.1073/pnas.0812071106

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

For most dsRNA viruses, the genome-enclosing capsid comprises 120 copies of a single capsid protein (CP) organized into 60 icosahedrally equivalent dimers, generally identified as 2 nonsymmetricallyinteracting CP molecules with extensive lateral contacts. The crystal structure of a partitivirus, Penicillium stoloniferum virus F (PsV-F), reveals a different organization, in which the CP dimer is related by almost-perfect local 2-fold symmetry, forms prominent surface arches, and includes extensive structure swapping between the 2 subunits. An electron cryomicroscopy map of PsV-F shows that the disordered N terminus of each CP molecule interacts with the dsRNA genome and probably participates in its packaging or transcription. Intact PsV-F particles mediate semiconservative transcription, and transcripts are likely to exit through negatively charged channels at the icosahedral 5-fold axes. Other findings suggest that the PsV-F capsid is assembled from dimers of CP dimers, with an arrangement similar to flavivirus E glycoproteins.

Bibliography

Pan, J., Dong, L., Lin, L., Ochoa, W. F., Sinkovits, R. S., Havens, W. M., Nibert, M. L., Baker, T. S., Ghabrial, S. A., & Tao, Y. J. (2009). Atomic structure reveals the unique capsid organization of a dsRNA virus. Proceedings of the National Academy of Sciences, 106(11), 4225â4230.

Authors 10

Junhua Pan (first)
Liping Dong (additional)
Li Lin (additional)
Wendy F. Ochoa (additional)
Robert S. Sinkovits (additional)
Wendy M. Havens (additional)
Max L. Nibert (additional)
Timothy S. Baker (additional)
Said A. Ghabrial (additional)
Yizhi Jane Tao (additional)

References 42 Referenced 78

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JW Kim, EY Choi, JI Lee, Genome organization and expression of the Penicillium stoloniferum virus F. Genes 31, 175–183 (2005). / Genes / Genome organization and expression of the Penicillium stoloniferum virus F by Kim JW (2005)
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Dates

Type	When
Created	16 years, 5 months ago (Feb. 25, 2009, 10:19 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 5:29 p.m.)
Indexed	4 weeks ago (July 25, 2025, 6:40 a.m.)
Issued	16 years, 5 months ago (March 17, 2009)
Published	16 years, 5 months ago (March 17, 2009)
Published Online	16 years, 5 months ago (March 17, 2009)
Published Print	16 years, 5 months ago (March 17, 2009)

Funders 0

None

BibTeX

@article{Pan_2009, title={Atomic structure reveals the unique capsid organization of a dsRNA virus}, volume={106}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0812071106}, DOI={10.1073/pnas.0812071106}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Pan, Junhua and Dong, Liping and Lin, Li and Ochoa, Wendy F. and Sinkovits, Robert S. and Havens, Wendy M. and Nibert, Max L. and Baker, Timothy S. and Ghabrial, Said A. and Tao, Yizhi Jane}, year={2009}, month=mar, pages={4225–4230} }

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