DOI: 10.1073/pnas.081079298. Similarities between the DNA replication initiators of Gram-negative bacteria plasmids (RepA) and eukaryotes (Orc4p)/archaea (Cdc6p)

10.1073/pnas.081079298

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The proteins responsible for the initiation of DNA replication are thought to be essentially unrelated in bacteria and archaea/eukaryotes. Here we show that RepA, the initiator from the Pseudomonas plasmid pPS10, and the C-terminal domain of ScOrc4p, a subunit of Saccharomyces cerevisiae (Sc) origin recognition complex (ORC), share sequence similarities. Based on biochemical and spectroscopic evidence, these similarities include common structural elements, such as a winged-helix domain and a leucine-zipper dimerization motif. We have also found that ScOrc4p, as previously described for RepA-type initiators, interacts with chaperones of the Hsp70 family both in vitro and in vivo , most probably to regulate the assembly of active ORC. In evolutionary terms, our results are compatible with the recruitment of the same protein module for initiation of DNA replication by the ancestors of present-day Gram-negative bacteria plasmids, archaea, and eukaryotes.

Bibliography

Giraldo, R., & DÃaz-Orejas, R. (2001). Similarities between the DNA replication initiators of Gram-negative bacteria plasmids (RepA) and eukaryotes (Orc4p)/archaea (Cdc6p). Proceedings of the National Academy of Sciences, 98(9), 4938â4943.

Authors 2

Rafael Giraldo (first)
Ramón Díaz-Orejas (additional)

References 42 Referenced 37

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:44 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 6:09 p.m.)
Indexed	2 months ago (June 20, 2025, 7:43 p.m.)
Issued	24 years, 4 months ago (April 10, 2001)
Published	24 years, 4 months ago (April 10, 2001)
Published Online	24 years, 4 months ago (April 10, 2001)
Published Print	24 years, 3 months ago (April 24, 2001)

Funders 0

None

BibTeX

@article{Giraldo_2001, title={Similarities between the DNA replication initiators of Gram-negative bacteria plasmids (RepA) and eukaryotes (Orc4p)/archaea (Cdc6p)}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.081079298}, DOI={10.1073/pnas.081079298}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Giraldo, Rafael and Díaz-Orejas, Ramón}, year={2001}, month=apr, pages={4938–4943} }

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