DOI: 10.1073/pnas.081078898. Evidence for two active branches for electron transfer in photosystem I

Evidence for two active branches for electron transfer in photosystem I

10.1073/pnas.081078898

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

All photosynthetic reaction centers share a common structural theme. Two related, integral membrane polypeptides sequester electron transfer cofactors into two quasi-symmetrical branches, each of which incorporates a quinone. In type II reaction centers [photosystem (PS) II and proteobacterial reaction centers], electron transfer proceeds down only one of the branches, and the mobile quinone on the other branch is used as a terminal acceptor. PS I uses iron-sulfur clusters as terminal acceptors, and the quinone serves only as an intermediary in electron transfer. Much effort has been devoted to understanding the unidirectionality of electron transport in type II reaction centers, and it was widely thought that PS I would share this feature. We have tested this idea by examiningin vivokinetics of electron transfer from the quinone in mutant PS I reaction centers. This transfer is associated with two kinetic components, and we show that mutation of a residue near the quinone in one branch specifically affects the faster component, while the corresponding mutation in the other branch specifically affects the slower component. We conclude that both electron transfer branches in PS I are active.

Authors 5

Mariana Guergova-Kuras (first)
Brent Boudreaux (additional)
Anne Joliot (additional)
Pierre Joliot (additional)
Kevin Redding (additional)

References 31 Referenced 302

10.1016/S0005-2728(96)00112-0
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10.1016/0005-2728(93)90030-J
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10.1021/bi990857c
10.1007/BF00015559
K Redding, F MacMillan, W Leibl, K Brettel, A W Rutherford, J Breton, J-D Rochaix Photosynthesis: Mechanisms and Effects, ed G Garab (Kluwer, Dordrecht, The Netherlands), pp. 591–594 (1998). (10.1007/978-94-011-3953-3_139) / Photosynthesis: Mechanisms and Effects by Redding K (1998)
10.1007/BF00021484
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10.1016/0092-8674(89)90939-2
10.1063/1.1149566
E H Harris The Chlamydomonas Sourcebook: A Comprehensive Guide to Biology and Laboratory Use (Academic, San Diego, 1989). / The Chlamydomonas Sourcebook: A Comprehensive Guide to Biology and Laboratory Use by Harris E H (1989)
10.1007/BF00183531
10.1006/jmbi.1997.1269
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R Delosme Photosynth Res 29, 45–54 (1991). (10.1007/BF00035205) / Photosynth Res by Delosme R (1991)
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W Nitschke, T Matteoli, A W Rutherford Origin and Evolution of Biological Energy Conversion, ed M Baltcheffsky (VCH, New York), pp. 177–203 (1996). / Origin and Evolution of Biological Energy Conversion by Nitschke W (1996)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:37 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:17 a.m.)
Indexed	2 days, 20 hours ago (Aug. 29, 2025, 5:54 a.m.)
Issued	24 years, 5 months ago (March 27, 2001)
Published	24 years, 5 months ago (March 27, 2001)
Published Online	24 years, 5 months ago (March 27, 2001)
Published Print	24 years, 4 months ago (April 10, 2001)

Funders 0

None

BibTeX

@article{Guergova_Kuras_2001, title={Evidence for two active branches for electron transfer in photosystem I}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.081078898}, DOI={10.1073/pnas.081078898}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Guergova-Kuras, Mariana and Boudreaux, Brent and Joliot, Anne and Joliot, Pierre and Redding, Kevin}, year={2001}, month=mar, pages={4437–4442} }

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